{"id":"6f339845-2ae1-5b94-a826-a11c1c9e533e","stable_key":"cb41aa31-e26b-5b60-b7e0-e4985fb7aea8:hydroxytyrosol-ampk-pink1-mitophagy","predicate":"blocks","statement":"Compound C blocked hydroxytyrosol-associated PINK1 mitophagy in zebrafish liver cells, and cyclosporine blocked the measured mitochondrial benefits.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8a53d7b2-dfb6-5da8-8eeb-deef67adb4dd","mechanism_event_label":"Compound C blocked hydroxytyrosol-associated PINK1 mitophagy in zebrafish liver cells, and cyclosporine blocked the measured mitochondrial benefits.","subject":{"id":"599ac23b-aba7-58a7-89e3-254ebb938852","slug":"compound-c-ampk-inhibitor","display_name":"Compound C AMPK inhibitor","entity_type_key":"drug"},"object":{"id":"0a0d8945-902a-5f5f-9f81-df1de48b572e","slug":"hydroxytyrosol-pink1-mitophagy","display_name":"Hydroxytyrosol-associated PINK1-mediated mitophagy","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8a53d7b2-dfb6-5da8-8eeb-deef67adb4dd","stable_key":"cb41aa31-e26b-5b60-b7e0-e4985fb7aea8:hydroxytyrosol-ampk-pink1-mitophagy-event","event_type":"observed_relationship","label":"Compound C blocked hydroxytyrosol-associated PINK1 mitophagy in zebrafish liver cells, and cyclosporine blocked the measured mitochondrial benefits.","description":"Compound C blocked hydroxytyrosol-associated PINK1 mitophagy in zebrafish liver cells, and cyclosporine blocked the measured mitochondrial benefits.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"599ac23b-aba7-58a7-89e3-254ebb938852","slug":"compound-c-ampk-inhibitor","display_name":"Compound C AMPK inhibitor","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0a0d8945-902a-5f5f-9f81-df1de48b572e","slug":"hydroxytyrosol-pink1-mitophagy","display_name":"Hydroxytyrosol-associated PINK1-mediated mitophagy","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"45a7aaec-8acc-5d5a-a014-c483ef5385e2","slug":"hydroxytyrosol","display_name":"Hydroxytyrosol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8128eec0-61cc-51ef-8d4e-5ec9678e8dfa","slug":"ampk-complexes","display_name":"AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"06dc6233-4a06-5804-a45a-3358ceae8b75","slug":"pink1","display_name":"Human PINK1 kinase","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"b199063a-fae6-5c5c-9cb4-22c10453bbac","slug":"cyclosporine","display_name":"Cyclosporine","entity_type_key":"drug"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"dose","value_text":"Hydroxytyrosol with compound C or cyclosporine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Cell assay accompanying fish feeding","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Zebrafish liver cell line with an eight-week spotted-seabass feeding arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This fish/cell mechanism is not direct evidence for human liver disease or a clinical drug interaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hydroxytyrosol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hydroxytyrosol","display_name":"Hydroxytyrosol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Zebrafish liver cell line with an eight-week spotted-seabass feeding arm","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Compound C blocked hydroxytyrosol-associated PINK1 mitophagy in zebrafish liver cells, and cyclosporine blocked the measured mitochondrial benefits.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Hydroxytyrosol Promotes the Mitochondrial Function through Activating Mitophagy. (2022). https://pubmed.ncbi.nlm.nih.gov/35624756/ DOI: 10.3390/antiox11050893","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro perturbation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"AMPK/PINK1 mitophagy and mitochondrial function","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"d3ed27fc-60ce-56af-a190-7481f20154e6","evidence_kind":"source_excerpt","locator":"Lines 121-130","start_line":121,"end_line":130,"excerpt":"## hydroxytyrosol-ampk-pink1-mitophagy\nCompound C blocked hydroxytyrosol-associated PINK1 mitophagy in zebrafish liver cells, and cyclosporine blocked the measured mitochondrial benefits.\nModel/species: Zebrafish liver cell line with an eight-week spotted-seabass feeding arm\nTissue/system: AMPK/PINK1 mitophagy and mitochondrial function\nExposure: Hydroxytyrosol with compound C or cyclosporine\nRoute: In vitro perturbation\nDuration: Cell assay accompanying fish feeding\nLimits: This fish/cell mechanism is not direct evidence for human liver disease or a clinical drug interaction.\nPrimary reference: Hydroxytyrosol Promotes the Mitochondrial Function through Activating Mitophagy. (2022). https://pubmed.ncbi.nlm.nih.gov/35624756/ DOI: 10.3390/antiox11050893\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Zebrafish liver cell line with an eight-week spotted-seabass feeding arm","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f83cd982-34f0-55d4-8563-eedfb0f009f2","stable_key":"import-cb41aa31-e26b-5b60-b7e0-e4985fb7aea8","title":"Hydroxytyrosol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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