{"id":"6f1cb5cd-bfe7-5d15-9b31-91993059e9d8","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-trxr-growth-requirement","predicate":"is_required_in_assay_for","statement":"Recovery of thioredoxin reductase activity over four days paralleled the return of the cells to a normal rate of growth, and the authors conclude that full activity of TrxR1, required for production of deoxyribonucleotides for DNA synthesis, is essential for normal growth of oxygen-challenged cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"98642f3e-5d93-5b9e-a588-243dfc4cc4cf","mechanism_event_label":"The cells could not start dividing again until that enzyme came back.","subject":{"id":"a340f08f-e7d6-5d4f-85b8-c5ea50d4d4e3","slug":"thioredoxin-reductase-activity","display_name":"Thioredoxin reductase catalytic activity","entity_type_key":"cellular_process"},"object":{"id":"0aa48629-f56b-5e79-9149-b93efe54b03f","slug":"lens-epithelial-cell-growth","display_name":"Human lens epithelial cell growth","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"98642f3e-5d93-5b9e-a588-243dfc4cc4cf","stable_key":"488ea171-36b8-5ef6-b5d3-98563980fdbf:hbot-trxr-growth-requirement-event","event_type":"biochemical_relationship","label":"The cells could not start dividing again until that enzyme came back.","description":"Recovery of thioredoxin reductase activity over four days paralleled the return of the cells to a normal rate of growth, and the authors conclude that full activity of TrxR1, required for production of deoxyribonucleotides for DNA synthesis, is essential for normal growth of oxygen-challenged cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2a9ca12-8d86-5da7-91f9-fd961c3b48b3","slug":"deoxyribonucleotide-synthesis","display_name":"Deoxyribonucleotide synthesis","entity_type_key":"cellular_process"},"role":"downstream_process","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"64fac263-c4d5-51c8-beba-d061868b0ce2","slug":"txnrd1","display_name":"TXNRD1","entity_type_key":"protein"},"role":"affected_enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a340f08f-e7d6-5d4f-85b8-c5ea50d4d4e3","slug":"thioredoxin-reductase-activity","display_name":"Thioredoxin reductase catalytic activity","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0aa48629-f56b-5e79-9149-b93efe54b03f","slug":"lens-epithelial-cell-growth","display_name":"Human lens epithelial cell growth","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/hbot-research/15642322.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e3bc035a17e4e19dd92d4fadb1a79f8e3801619dac24fb91dcf985728afaf995\", \"start_char\": 0, \"end_char\": 2646, \"text_sha256\": \"e3bc035a17e4e19dd92d4fadb1a79f8e3801619dac24fb91dcf985728afaf995\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured human lens epithelial cells exposed to extreme hyperbaric oxygen with enzyme assays and real-time PCR","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"99% oxygen at 50 atmospheres for 3 hours, then normal culture for up to 11 days","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Fifty atmospheres is an extreme experimental exposure far above therapy, chosen to probe which defences matter. The selenoenzyme result is the informative part; the pressure is not clinically relevant.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hyperbaric oxygen research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hyperbaric-oxygen","display_name":"Hyperbaric oxygen therapy","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Human cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The cells could not start dividing again until that enzyme came back.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[hbot-p15642322] Thioredoxin reductase may be essential for the normal growth of hyperbaric oxygen-treated human lens epithelial cells. (2004). https://pubmed.ncbi.nlm.nih.gov/15642322/ DOI: 10.1016/j.exer.2004.07.001","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Lens epithelium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"82795e18-26a4-5a03-8ae1-add9bac55d5d","evidence_kind":"source_excerpt","locator":"Lines 400-411","start_line":400,"end_line":411,"excerpt":"### hbot-trxr-growth-requirement\nRecovery of thioredoxin reductase activity over four days paralleled the return of the cells to a normal rate of growth, and the authors conclude that full activity of TrxR1, required for production of deoxyribonucleotides for DNA synthesis, is essential for normal growth of oxygen-challenged cells.\nCondition category: machinery_impairment\nnutrient_topic: Hyperbaric oxygen research collection; topical membership is not evidence of a direct clinical effect, and a therapeutic exposure is not a dietary intake.\nplain_language: The cells could not start dividing again until that enzyme came back.\norganism: Human cells\ntissue_or_cell_type: Lens epithelium\nexperimental_model: Cultured human lens epithelial cells exposed to extreme hyperbaric oxygen with enzyme assays and real-time PCR\nlimitations: Fifty atmospheres is an extreme experimental exposure far above therapy, chosen to probe which defences matter. The selenoenzyme result is the informative part; the pressure is not clinically relevant.\nexposure: 99% oxygen at 50 atmospheres for 3 hours, then normal culture for up to 11 days\nevidence_span: {\"source_cache\": \"artifacts/hbot-research/15642322.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e3bc035a17e4e19dd92d4fadb1a79f8e3801619dac24fb91dcf985728afaf995\", \"start_char\": 0, \"end_char\": 2646, \"text_sha256\": \"e3bc035a17e4e19dd92d4fadb1a79f8e3801619dac24fb91dcf985728afaf995\"}\n[hbot-p15642322] Thioredoxin reductase may be essential for the normal growth of hyperbaric oxygen-treated human lens epithelial cells. (2004). https://pubmed.ncbi.nlm.nih.gov/15642322/ DOI: 10.1016/j.exer.2004.07.001","model_system":"Cultured human lens epithelial cells exposed to extreme hyperbaric oxygen with enzyme assays and real-time PCR","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [hbot-p15642322] Thioredoxin reductase may be essential for the normal growth of hyperbaric oxygen-treated human lens epithelial cells. (2004). https://pubmed.ncbi.nlm.nih.gov/15642322/ DOI: 10.1016/j.exer.2004.07.001","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dbf39b48-c95e-5291-b404-349ffbc4488f","stable_key":"import-488ea171-36b8-5ef6-b5d3-98563980fdbf","title":"Hyperbaric oxygen: the exposure, its reactive species, the signals they carry, and the nutrient-dependent enzymes that handle them (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"fb2443321804010d82c6b96ab61cc51b4b6628ddbb15751cb0e90b491606e79c","revision_id":"bb699ea5-b492-5728-b22c-48f0842a8ac9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}