{"id":"6f03530d-63db-589d-a8e1-5085ada9ddc2","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-piperine-shared-trpv1","predicate":"shares_agonist_and_desensitization_target_with","statement":"Piperine and capsaicin both stimulated TRPV1-dependent calcium responses in human PC-3 cells and both showed reduced responses on second exposure.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"40c7eecd-3604-5c19-a017-99e1f0084bd2","mechanism_event_label":"Piperine and capsaicin both stimulated TRPV1-dependent calcium responses in human PC-3 cells and both showed reduced responses on second exposure.","subject":{"id":"4d1060b9-e9b4-5ffd-88c2-21362c5d8491","slug":"piperine","display_name":"Piperine","entity_type_key":"small_molecule"},"object":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"40c7eecd-3604-5c19-a017-99e1f0084bd2","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-piperine-shared-trpv1-event","event_type":"observed_relationship","label":"Piperine and capsaicin both stimulated TRPV1-dependent calcium responses in human PC-3 cells and both showed reduced responses on second exposure.","description":"Piperine and capsaicin both stimulated TRPV1-dependent calcium responses in human PC-3 cells and both showed reduced responses on second exposure.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4d1060b9-e9b4-5ffd-88c2-21362c5d8491","slug":"piperine","display_name":"Piperine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"514451f2-5b73-501c-a004-07c1f7895cbd","slug":"sb366791","display_name":"SB366791 TRPV1 antagonist","entity_type_key":"drug"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Capsaicin or piperine concentration-response; second exposure; SB366791 control","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute sequential exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human PC-3 cells expressing TRPV1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Comparable assay behavior does not establish equivalent potency, oral exposure, or benefit in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Capsaicin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human PC-3 cells expressing TRPV1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Piperine and capsaicin both stimulated TRPV1-dependent calcium responses in human PC-3 cells and both showed reduced responses on second exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pharmacodynamics of TRPV1 agonists in a bioassay using human PC-3 cells. (2014). https://pubmed.ncbi.nlm.nih.gov/24688365/ DOI: 10.1155/2014/184526","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Fluo-4 calcium-response bioassay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b2dc1546-4199-5fc2-b623-fe062553047f","evidence_kind":"source_excerpt","locator":"Lines 110-119","start_line":110,"end_line":119,"excerpt":"## capsaicin-piperine-shared-trpv1\nPiperine and capsaicin both stimulated TRPV1-dependent calcium responses in human PC-3 cells and both showed reduced responses on second exposure.\nModel/species: Human PC-3 cells expressing TRPV1\nTissue/system: Fluo-4 calcium-response bioassay\nExposure: Capsaicin or piperine concentration-response; second exposure; SB366791 control\nRoute: In vitro\nDuration: Acute sequential exposure\nLimits: Comparable assay behavior does not establish equivalent potency, oral exposure, or benefit in humans.\nPrimary reference: Pharmacodynamics of TRPV1 agonists in a bioassay using human PC-3 cells. (2014). https://pubmed.ncbi.nlm.nih.gov/24688365/ DOI: 10.1155/2014/184526\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Human PC-3 cells expressing TRPV1","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"883c8afb-af48-5561-ae3b-00d4f3c26752","stable_key":"import-49fecf36-4958-5f15-8c78-9a68cba50763","title":"Capsaicin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"ea5c8dc49ffdfcec3f5542fed8be406c4af37873b76416910f88a743b733466e","revision_id":"79e49bef-520e-5503-ad2e-7de89ad236cf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}