{"id":"6eead2ce-8cd3-518f-a62f-67585ef7c590","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:pituitary-tsh-up","predicate":"increases_in_recorded_experiment","statement":"Thymulin increased TSH release in a separate rat pituitary experiment; TRH and cAMP-associated interventions potentiated responses and EGTA blocked release.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"96531b2c-9ca6-5805-ad47-7b23b0997ef6","mechanism_event_label":"Thymulin increased TSH release in a separate rat pituitary experiment; TRH and cAMP-associated interventions potentiated responses and EGTA blocked release.","subject":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"object":{"id":"4e761476-7055-5afd-9be1-b6d2ae14e2f8","slug":"rat-pituitary-tsh-release","display_name":"Rat anterior-pituitary TSH release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"96531b2c-9ca6-5805-ad47-7b23b0997ef6","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:pituitary-tsh-up-event","event_type":"experimental_observation","label":"Thymulin increased TSH release in a separate rat pituitary experiment; TRH and cAMP-associated interventions potentiated responses and EGTA blocked release.","description":"Likewise, a separate study found **prolactin and TSH increases**, with a bell-shaped concentration response in incubation experiments, potentiation by TRH and cAMP-associated interventions, and weaker responses in senescent preparations. EGTA blocked release. The opposite prolactin directions across studies are an unresolved comparison involving concentration and preparation, not grounds to discard either result. TRH-like behavior does not establish binding to the TRH receptor. [1998 prolactin/TSH study](https://pubmed.ncbi.nlm.nih.gov/9818729/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"tested factor","stoichiometry":null,"state_label":"Thymulin","sequence_order":0,"notes":""},{"entity":{"id":"4e761476-7055-5afd-9be1-b6d2ae14e2f8","slug":"rat-pituitary-tsh-release","display_name":"Rat anterior-pituitary TSH release","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"calcium-sensitive signaling","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary bibliographic record and abstract checked; full methods/figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Thymulin\", \"comparator\": \"Matched pituitary preparation\", \"endpoint\": \"Thymulin increased TSH release in a separate rat pituitary experiment; TRH and cAMP-associated interventions potentiated responses and EGTA blocked release.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat pituitary incubation study; concentration/age dependence, not demonstrated binding to the TRH receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Thymulin increased TSH release in a separate rat pituitary experiment; TRH and cAMP-associated interventions potentiated responses and EGTA blocked release.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Thymulin stimulates prolactin and thyrotropin release in an age-related manner. | 1998 | DOI 10.1016/s0047-6374(98)00072-4 | PMID 9818729 | https://pubmed.ncbi.nlm.nih.gov/9818729/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 108-108; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6fa81b3d-00ba-5e49-a386-ee563d6564f2","evidence_kind":"source_excerpt","locator":"Lines 108-108","start_line":108,"end_line":108,"excerpt":"Likewise, a separate study found **prolactin and TSH increases**, with a bell-shaped concentration response in incubation experiments, potentiation by TRH and cAMP-associated interventions, and weaker responses in senescent preparations. EGTA blocked release. The opposite prolactin directions across studies are an unresolved comparison involving concentration and preparation, not grounds to discard either result. TRH-like behavior does not establish binding to the TRH receptor. [1998 prolactin/TSH study](https://pubmed.ncbi.nlm.nih.gov/9818729/)","model_system":"Rat pituitary incubation study; concentration/age dependence, not demonstrated binding to the TRH receptor.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e1871d49-9c6b-547b-a1bb-6e2b9270ca89","stable_key":"import-18100170-a014-51c3-adeb-9dab4162b8cc","title":"Thymulin: detailed mechanisms of action (reviewed 3 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"6e8ed0d7a5e23fc822f2ab418e0c4fb2411c01fcaa330039e8d7d93218cacc88","revision_id":"8c0e3449-9bbd-57c0-bb5e-de83f044f2ac","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}