{"id":"6ed99973-1479-5bf2-87ec-b617537866fa","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:mouse-hematology","predicate":"increases_in_recorded_experiment","statement":"Small neutrophil, eosinophil and hematocrit increases occurred in the high-dose feeding group.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"5418c1b6-d627-52f5-9697-021220a1e648","mechanism_event_label":"Small neutrophil, eosinophil and hematocrit increases occurred in the high-dose feeding group.","subject":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"object":{"id":"a00ef1c4-c17d-5c86-a2b1-9c29b51115e6","slug":"mouse-sa-feeding-hematology","display_name":"Mouse hematological changes after shikimic-acid feeding","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5418c1b6-d627-52f5-9697-021220a1e648","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:mouse-hematology-event","event_type":"experimental_observation","label":"Small neutrophil, eosinophil and hematocrit increases occurred in the high-dose feeding group.","description":"**Mouse distribution and bounded toxicity observations.** The 2025 mouse study reported intravenous half-lives approximately 0.76–0.85 h at 4–16 mg/kg and oral bioavailability approximately 11.09–20.44% at 50–100 mg/kg. A short plasma half-life does not prove unchanged renal excretion. In a separate 28-day feeding experiment, dietary concentrations were 5.56, 16.67 and 50 g/kg FEED, not mg/kg body weight. High-dose animals had small hematological shifts and lower triglycerides; acute survival through tested doses is not evidence of lifetime human safety. No validated human monocarboxylate-transporter assignment was found in the reviewed sources. [Pharmacokinetic Profile and Evaluation of Acute and Subchronic Oral Toxicity of Shikimic Acid in Mice.](https://pubmed.ncbi.nlm.nih.gov/40852254/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"High dietary shikimic-acid concentration","sequence_order":0,"notes":""},{"entity":{"id":"a00ef1c4-c17d-5c86-a2b1-9c29b51115e6","slug":"mouse-sa-feeding-hematology","display_name":"Mouse hematological changes after shikimic-acid feeding","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"High dietary shikimic-acid concentration\", \"comparator\": \"Control feed\", \"endpoint\": \"Small neutrophil, eosinophil and hematocrit increases occurred in the high-dose feeding group.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"ICR mice; 28-day feeding study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Measured shifts do not establish beneficial immunity or long-term safety.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Small neutrophil, eosinophil and hematocrit increases occurred in the high-dose feeding group.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pharmacokinetic Profile and Evaluation of Acute and Subchronic Oral Toxicity of Shikimic Acid in Mice. | 2025 | DOI 10.1021/acsomega.5c03740 | PMID 40852254 | https://pubmed.ncbi.nlm.nih.gov/40852254/ | https://doi.org/10.1021/acsomega.5c03740 | https://pmc.ncbi.nlm.nih.gov/articles/PMC12368620/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 51-51; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5ab92fbb-96be-5d62-a192-2f88900ac233","evidence_kind":"source_excerpt","locator":"Lines 51-51","start_line":51,"end_line":51,"excerpt":"**Mouse distribution and bounded toxicity observations.** The 2025 mouse study reported intravenous half-lives approximately 0.76–0.85 h at 4–16 mg/kg and oral bioavailability approximately 11.09–20.44% at 50–100 mg/kg. A short plasma half-life does not prove unchanged renal excretion. In a separate 28-day feeding experiment, dietary concentrations were 5.56, 16.67 and 50 g/kg FEED, not mg/kg body weight. High-dose animals had small hematological shifts and lower triglycerides; acute survival through tested doses is not evidence of lifetime human safety. No validated human monocarboxylate-transporter assignment was found in the reviewed sources. [Pharmacokinetic Profile and Evaluation of Acute and Subchronic Oral Toxicity of Shikimic Acid in Mice.](https://pubmed.ncbi.nlm.nih.gov/40852254/)","model_system":"ICR mice; 28-day feeding study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e6ae59de-0369-5c2f-8262-57d91302671c","stable_key":"import-31b1baa4-4113-5541-b9e7-fe44a5253a07","title":"Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"95b1f9e9577661312d67f36e156d2e49326b207f296c1c0e801a5b007fd8e283","revision_id":"cd3237f1-131a-557d-84b5-7543259807b0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}