{"id":"6ecfc492-8218-57a6-b408-29d7b11be41e","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:bv2-mouse-tnf-transcript","predicate":"decreases_in_recorded_experiment","statement":"Shikimic acid reduced the LPS-associated transcript readout for mouse-tnf in BV2 cells.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"fa9804b0-ddd7-50b6-82bd-e9a84622ebc4","mechanism_event_label":"Shikimic acid reduced the LPS-associated transcript readout for mouse-tnf in BV2 cells.","subject":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"object":{"id":"01c46821-30eb-50b2-9c65-fd50ebedfe70","slug":"mouse-tnf","display_name":"Mouse tumor necrosis factor / Tnf","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fa9804b0-ddd7-50b6-82bd-e9a84622ebc4","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:bv2-mouse-tnf-transcript-event","event_type":"experimental_observation","label":"Shikimic acid reduced the LPS-associated transcript readout for mouse-tnf in BV2 cells.","description":"**Inflammatory expression and phosphorylation are distinct endpoints.** In the same BV2/LPS model, shikimic acid reduced Il6, Tnf, Nos2 and Ptgs2 transcripts at 12 h; protein/secretion measurements at 24 h also decreased. At 1 h, p65 and IκB phosphorylation decreased and IκB degradation was reduced. These changes do not establish direct inhibition of COX-2 catalysis or direct binding to every signaling protein. [Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced <i>in vitro</i> and <i>in vivo</i> model.](https://pubmed.ncbi.nlm.nih.gov/38026972/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"SA plus LPS","sequence_order":0,"notes":""},{"entity":{"id":"01c46821-30eb-50b2-9c65-fd50ebedfe70","slug":"mouse-tnf","display_name":"Mouse tumor necrosis factor / Tnf","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"e96aad69-74fb-55c6-8378-7d5c281003eb","slug":"lipopolysaccharide","display_name":"Lipopolysaccharide","entity_type_key":"chemical_species"},"role":"inflammatory stimulus","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"stimulus","comparator":"LPS without SA","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"lipopolysaccharide","display_name":"Lipopolysaccharide","entity_type_key":"chemical_species"}},{"dimension":"experimental_condition","value_text":"added","comparator":"LPS without SA","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"SA plus LPS\", \"comparator\": \"LPS without SA\", \"endpoint\": \"Shikimic acid reduced the LPS-associated transcript readout for mouse-tnf in BV2 cells.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"shikimic-acid\", \"state\": \"added\"}, {\"entity_slug\": \"lipopolysaccharide\", \"state\": \"stimulus\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Mouse BV2: 5/10 µM SA plus LPS 1 µg/mL; transcript at 12 h, protein/secretion at 24 h.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression or secretion endpoint; not direct enzyme catalytic inhibition, binding or a human response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Shikimic acid reduced the LPS-associated transcript readout for mouse-tnf in BV2 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced <i>in vitro</i> and <i>in vivo</i> model. | 2023 | DOI 10.3389/fphar.2023.1265571 | PMID 38026972 | https://pubmed.ncbi.nlm.nih.gov/38026972/ | https://doi.org/10.3389/fphar.2023.1265571 | https://pmc.ncbi.nlm.nih.gov/articles/PMC10652795/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 63-63; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dac23aa8-936b-5aa0-92e6-3e8c7f4a8f6e","evidence_kind":"source_excerpt","locator":"Lines 63-63","start_line":63,"end_line":63,"excerpt":"**Inflammatory expression and phosphorylation are distinct endpoints.** In the same BV2/LPS model, shikimic acid reduced Il6, Tnf, Nos2 and Ptgs2 transcripts at 12 h; protein/secretion measurements at 24 h also decreased. At 1 h, p65 and IκB phosphorylation decreased and IκB degradation was reduced. These changes do not establish direct inhibition of COX-2 catalysis or direct binding to every signaling protein. [Shikimic acid (SA) inhibits neuro-inflammation and exerts neuroprotective effects in an LPS-induced <i>in vitro</i> and <i>in vivo</i> model.](https://pubmed.ncbi.nlm.nih.gov/38026972/)","model_system":"Mouse BV2: 5/10 µM SA plus LPS 1 µg/mL; transcript at 12 h, protein/secretion at 24 h.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e6ae59de-0369-5c2f-8262-57d91302671c","stable_key":"import-31b1baa4-4113-5541-b9e7-fe44a5253a07","title":"Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"95b1f9e9577661312d67f36e156d2e49326b207f296c1c0e801a5b007fd8e283","revision_id":"cd3237f1-131a-557d-84b5-7543259807b0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}