{"id":"6eb5d972-34cb-594b-88bb-65d7c1c2ca2e","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-carns1-cell-protection","predicate":"overexpression_associated_with","statement":"Stable carnosine-synthase expression in human SH-SY5Y cells was associated with 2-oxo-carnosine formation and less peroxide cytotoxicity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7d6298a6-8600-581d-ae3f-2fa1346bc059","mechanism_event_label":"Increasing synthesis changed the cellular response to an oxidant.","subject":{"id":"dda068ca-005f-50f4-9001-42fe79759324","slug":"human-carns1","display_name":"Human carnosine synthase / CARNS1 (ATPGD1)","entity_type_key":"protein"},"object":{"id":"baed0602-7086-5f58-8766-05750afb8ce8","slug":"human-shsy5y-peroxide-survival","display_name":"Human SH-SY5Y cell survival under hydrogen peroxide","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7d6298a6-8600-581d-ae3f-2fa1346bc059","stable_key":"ae1ed35d-8ce7-5936-9067-a641374bbf79:carnosine-carns1-cell-protection-event","event_type":"observed_relationship","label":"Increasing synthesis changed the cellular response to an oxidant.","description":"Stable carnosine-synthase expression in human SH-SY5Y cells was associated with 2-oxo-carnosine formation and less peroxide cytotoxicity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dda068ca-005f-50f4-9001-42fe79759324","slug":"human-carns1","display_name":"Human carnosine synthase / CARNS1 (ATPGD1)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"baed0602-7086-5f58-8766-05750afb8ce8","slug":"human-shsy5y-peroxide-survival","display_name":"Human SH-SY5Y cell survival under hydrogen peroxide","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9998eb9b-0d09-58c9-885b-a442477dec8d","slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3ac09be9-1667-59a0-aaaa-ddb68c60be24","slug":"2-oxo-carnosine","display_name":"2-Oxo-carnosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human neuroblastoma expression model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not isolate 2-oxo-carnosine as the only protective mediator.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Carnosine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"carnosine","display_name":"L-Carnosine / beta-alanyl-L-histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Increasing synthesis changed the cellular response to an oxidant.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"2-Oxo-histidine-containing dipeptides are functional oxidation products. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30504220/ · DOI 10.1074/jbc.RA118.006111","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d6472ca6-20ef-5906-a2f4-8f6bda66fb9d","evidence_kind":"source_excerpt","locator":"Lines 348-354","start_line":348,"end_line":354,"excerpt":"## carnosine-carns1-cell-protection\nIncreasing synthesis changed the cellular response to an oxidant.\nStable carnosine-synthase expression in human SH-SY5Y cells was associated with 2-oxo-carnosine formation and less peroxide cytotoxicity.\nModel: Human neuroblastoma expression model.\nLimitations: Does not isolate 2-oxo-carnosine as the only protective mediator.\nEvidence access: Primary abstract\n2-Oxo-histidine-containing dipeptides are functional oxidation products. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30504220/ · DOI 10.1074/jbc.RA118.006111","model_system":"Human neuroblastoma expression model.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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