{"id":"6e475e74-1a39-57bf-be2b-811b4ccf0283","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-srr-overexpression","predicate":"increases_tested","statement":"SRR overexpression increased intracellular D-aspartate in rat PC12 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4adb5912-160f-5c55-a140-bf5bc53dce8f","mechanism_event_label":"Adding more racemase increased the measured D-aspartate pool.","subject":{"id":"08aec6b5-36e7-52d8-87bd-c0bf7adc43fc","slug":"mammalian-srr-assay","display_name":"Mammalian serine-racemase assay, species unresolved in accessed abstract","entity_type_key":"protein"},"object":{"id":"6f410950-b5e0-57d2-8a42-5b754b138038","slug":"rat-pc12-d-aspartate-pool","display_name":"D-aspartate pool in rat PC12 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4adb5912-160f-5c55-a140-bf5bc53dce8f","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-srr-overexpression-event","event_type":"observed_relationship","label":"Adding more racemase increased the measured D-aspartate pool.","description":"SRR overexpression increased intracellular D-aspartate in rat PC12 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"08aec6b5-36e7-52d8-87bd-c0bf7adc43fc","slug":"mammalian-srr-assay","display_name":"Mammalian serine-racemase assay, species unresolved in accessed abstract","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6f410950-b5e0-57d2-8a42-5b754b138038","slug":"rat-pc12-d-aspartate-pool","display_name":"D-aspartate pool in rat PC12 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat PC12 host cells; overexpressed construct species not resolved here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Overexpression does not establish that native SRR is the sole source.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Adding more racemase increased the measured D-aspartate pool.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Serine racemase is involved in d-aspartate biosynthesis. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27387750/ · DOI 10.1093/jb/mvw043","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8f458e7b-f4e0-5111-a79f-6d207dc52aa8","evidence_kind":"source_excerpt","locator":"Lines 24-30","start_line":24,"end_line":30,"excerpt":"## d-aspartate-srr-overexpression\nAdding more racemase increased the measured D-aspartate pool.\nSRR overexpression increased intracellular D-aspartate in rat PC12 cells.\nModel: Rat PC12 host cells; overexpressed construct species not resolved here.\nLimitations: Overexpression does not establish that native SRR is the sole source.\nEvidence access: Primary abstract\nSerine racemase is involved in d-aspartate biosynthesis. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27387750/ · DOI 10.1093/jb/mvw043","model_system":"Rat PC12 host cells; overexpressed construct species not resolved here.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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