{"id":"6e264272-da0a-534f-aa51-1526bf88040d","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-cars2-translation","predicate":"supports_mitochondrial_cysteinyl_trna_function","statement":"CARS2 disruption reduced mitochondrial cysteinyl-tRNA synthetase function, assessed in part through mitochondrial MTCO1 expression; mutant rescue distinguished this from persulfide synthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d96b889c-544e-5759-a8da-7d72f148095d","mechanism_event_label":"Mitochondria need their own enzyme to place cysteine into proteins.","subject":{"id":"ea176dd8-338c-5b68-be84-5095521a20c7","slug":"cars2","display_name":"Human mitochondrial cysteinyl-tRNA synthetase / CARS2","entity_type_key":"protein"},"object":{"id":"3219cfb5-ce32-5cbb-b9a7-cf2bbfc99aed","slug":"human-mitochondrial-cysteine-translation","display_name":"Human mitochondrial cysteine incorporation during translation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d96b889c-544e-5759-a8da-7d72f148095d","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-cars2-translation-event","event_type":"observed_relationship","label":"Mitochondria need their own enzyme to place cysteine into proteins.","description":"CARS2 disruption reduced mitochondrial cysteinyl-tRNA synthetase function, assessed in part through mitochondrial MTCO1 expression; 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