{"id":"6dac1b4e-8d90-50f9-891e-10e32c2724b1","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-ryr-sensitive-calcium","predicate":"reduces","statement":"Boric acid reduced calcium release evoked by ryanodine-receptor agonists in DU-145 cells; cADPR inhibition was detected at 50 µM and responses to other agonists at different concentrations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"9f52b043-ad26-58e8-8823-80809756ee50","mechanism_event_label":"Boric acid changed release from a particular calcium store in these cells.","subject":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"object":{"id":"a9f15f93-b978-5e9c-b3e3-dbf1134ae40a","slug":"intracellular-calcium-release","display_name":"Intracellular calcium-store release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9f52b043-ad26-58e8-8823-80809756ee50","stable_key":"7edf94bb-95c8-5234-9161-9eb338bb9b36:boron-ryr-sensitive-calcium-event","event_type":"biochemical_relationship","label":"Boric acid changed release from a particular calcium store in these cells.","description":"Boric acid reduced calcium release evoked by ryanodine-receptor agonists in DU-145 cells; cADPR inhibition was detected at 50 µM and responses to other agonists at different concentrations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"68a41868-91b4-574d-bd2a-6af3af7dc6db","slug":"ryanodine-receptor-family","display_name":"Ryanodine receptor family / RyR (isoform unspecified)","entity_type_key":"protein_family"},"role":"channel_family","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"55ff303e-ae6b-5adf-b34f-a11a53812e9a","slug":"cyclic-adp-ribose","display_name":"Cyclic ADP-ribose","entity_type_key":"small_molecule"},"role":"agonist","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"56db2f3c-f247-573f-bb7c-47cac02ea60f","slug":"boric-acid","display_name":"Boric acid / orthoboric acid / B(OH)3","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a9f15f93-b978-5e9c-b3e3-dbf1134ae40a","slug":"intracellular-calcium-release","display_name":"Intracellular calcium-store release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/boron-research/19554099.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\", \"start_char\": 0, \"end_char\": 1809, \"text_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Calcium imaging and flow cytometry in three human prostate cell lines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Boric acid 1–150 µM depending on agonist and cell line; DU-145 storage assay 50 µM for 1 hour","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"RyR-sensitive flux is not proof of direct binding to a particular RyR isoform. Cell-line exposure responses do not establish dietary prevention of prostate cancer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Boron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"boron","display_name":"Boron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human DU-145, LNCaP and PWR1E cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Boric acid changed release from a particular calcium store in these cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[boron-p19554099] Receptor activated Ca(2+) release is inhibited by boric acid in prostate cancer cells. (2009). https://pubmed.ncbi.nlm.nih.gov/19554099/ DOI: 10.1371/journal.pone.0006009","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cancer and non-tumor prostate cell cultures","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"2958ed54-b753-538d-9501-a168148f6a0f","evidence_kind":"source_excerpt","locator":"Lines 326-337","start_line":326,"end_line":337,"excerpt":"### boron-ryr-sensitive-calcium\nBoric acid reduced calcium release evoked by ryanodine-receptor agonists in DU-145 cells; cADPR inhibition was detected at 50 µM and responses to other agonists at different concentrations.\nCondition category: normal\nnutrient_topic: Boron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Boric acid changed release from a particular calcium store in these cells.\norganism: Human DU-145, LNCaP and PWR1E cells\ntissue_or_cell_type: Cancer and non-tumor prostate cell cultures\nexperimental_model: Calcium imaging and flow cytometry in three human prostate cell lines\nlimitations: RyR-sensitive flux is not proof of direct binding to a particular RyR isoform. Cell-line exposure responses do not establish dietary prevention of prostate cancer.\nexposure: Boric acid 1–150 µM depending on agonist and cell line; DU-145 storage assay 50 µM for 1 hour\nevidence_span: {\"source_cache\": \"artifacts/boron-research/19554099.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\", \"start_char\": 0, \"end_char\": 1809, \"text_sha256\": \"17bd65bde36f54d1bb0688118212419b31643b9fc61323d12ef1ad6a78a173bb\"}\n[boron-p19554099] Receptor activated Ca(2+) release is inhibited by boric acid in prostate cancer cells. 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