{"id":"6da35e0a-04e0-5abc-956d-945b54fea806","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-catabolic-products","predicate":"bacterial_catabolism_produces","statement":"The reconstituted bacterial pathway converted ergothioneine into glutamate, trimethylamine, hydrogen sulfide, carbon dioxide and ammonia.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"dbb2beff-7506-5bf9-b73f-7c7718e69e31","mechanism_event_label":"The sulfur and carbon skeleton enter separate products.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"70cc3ced-8adc-5e76-9750-e1415683455b","slug":"hydrogen-sulfide","display_name":"Hydrogen sulfide / H2S","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"dbb2beff-7506-5bf9-b73f-7c7718e69e31","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-catabolic-products-event","event_type":"observed_relationship","label":"The sulfur and carbon skeleton enter separate products.","description":"The reconstituted bacterial pathway converted ergothioneine into glutamate, trimethylamine, hydrogen sulfide, carbon dioxide and ammonia.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"70cc3ced-8adc-5e76-9750-e1415683455b","slug":"hydrogen-sulfide","display_name":"Hydrogen sulfide / H2S","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ffdcd3fa-0e47-5c2b-96e2-00b7d1d5674e","slug":"trimethylamine","display_name":"Trimethylamine / TMA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"In vitro five-step bacterial enzyme system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Environmental gene distribution does not quantify human intestinal flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The sulfur and carbon skeleton enter separate products.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"In Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33544568/ · DOI 10.1021/acschembio.0c00968","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9de66db4-4eb6-5a85-beff-a053718adfa7","evidence_kind":"source_excerpt","locator":"Lines 272-278","start_line":272,"end_line":278,"excerpt":"## ergothioneine-catabolic-products\nThe sulfur and carbon skeleton enter separate products.\nThe reconstituted bacterial pathway converted ergothioneine into glutamate, trimethylamine, hydrogen sulfide, carbon dioxide and ammonia.\nModel: In vitro five-step bacterial enzyme system.\nLimitations: Environmental gene distribution does not quantify human intestinal flux.\nEvidence access: Primary abstract\nIn Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33544568/ · DOI 10.1021/acschembio.0c00968","model_system":"In vitro five-step bacterial enzyme system.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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