{"id":"6d715a91-e7eb-5046-ba38-b640919bb8ad","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-tryptamine-secretion","predicate":"increases","statement":"Tryptamine increased epithelial ionic flux and colonoid fluid secretion through 5-HT4 receptors in mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c6ac1fd4-9b71-5c0f-af39-47ee99caf1c1","mechanism_event_label":"A microbial tryptophan product can change intestinal fluid movement.","subject":{"id":"e009ad82-c5b9-5dba-96dd-b7e85060da2c","slug":"tryptamine","display_name":"Tryptamine","entity_type_key":"small_molecule"},"object":{"id":"850b1f47-62fb-535f-b1e1-3b250e7b6c25","slug":"mouse-tryptamine-secretion","display_name":"Mouse colonic ionic and fluid secretion after tryptamine","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c6ac1fd4-9b71-5c0f-af39-47ee99caf1c1","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-tryptamine-secretion-event","event_type":"observed_relationship","label":"A microbial tryptophan product can change intestinal fluid movement.","description":"Tryptamine increased epithelial ionic flux and colonoid fluid secretion through 5-HT4 receptors in mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e009ad82-c5b9-5dba-96dd-b7e85060da2c","slug":"tryptamine","display_name":"Tryptamine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"850b1f47-62fb-535f-b1e1-3b250e7b6c25","slug":"mouse-tryptamine-secretion","display_name":"Mouse colonic ionic and fluid secretion after tryptamine","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fd074f31-c694-5703-9237-0544bf024341","slug":"mouse-htr4","display_name":"Mouse serotonin receptor 4 / Htr4","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Germ-free and human-stool-colonized mouse tissue/colonoids.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Humanized microbiota does not make the host tissue human.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A microbial tryptophan product can change intestinal fluid movement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Gut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29902441/ · DOI 10.1016/j.chom.2018.05.004","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e682da5c-9c6a-554c-950a-002016750cdf","evidence_kind":"source_excerpt","locator":"Lines 562-568","start_line":562,"end_line":568,"excerpt":"## tryptophan-tryptamine-secretion\nA microbial tryptophan product can change intestinal fluid movement.\nTryptamine increased epithelial ionic flux and colonoid fluid secretion through 5-HT4 receptors in mice.\nModel: Germ-free and human-stool-colonized mouse tissue/colonoids.\nLimitations: Humanized microbiota does not make the host tissue human.\nEvidence access: Primary abstract\nGut Microbiota-Produced Tryptamine Activates an Epithelial G-Protein-Coupled Receptor to Increase Colonic Secretion. · 2018 · https://pubmed.ncbi.nlm.nih.gov/29902441/ · DOI 10.1016/j.chom.2018.05.004","model_system":"Germ-free and human-stool-colonized mouse tissue/colonoids.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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