{"id":"6d3d76e4-9ffb-5735-a79a-d3b107d21e60","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-brain-receptor-required","predicate":"knockout_blocks","statement":"Acetyl-L-carnitine did not produce the reported behavioral benefit in mGlu2-knockout mice exposed to chronic unpredictable stress.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ab3da620-7bcd-54fa-9e09-7821a1bcd3d1","mechanism_event_label":"Removing the receptor tested whether the rodent effect depended on it.","subject":{"id":"0cec7eb2-cb95-5751-a161-3d2a4033f22b","slug":"mouse-grm2","display_name":"Mouse metabotropic glutamate receptor 2 / Grm2","entity_type_key":"protein"},"object":{"id":"964f7af1-e158-5cf7-9874-a9be7501ced7","slug":"mouse-alc-stress-behavior","display_name":"Mouse stress-model behavioral response to acetylcarnitine","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ab3da620-7bcd-54fa-9e09-7821a1bcd3d1","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-brain-receptor-required-event","event_type":"observed_relationship","label":"Removing the receptor tested whether the rodent effect depended on it.","description":"Acetyl-L-carnitine did not produce the reported behavioral benefit in mGlu2-knockout mice exposed to chronic unpredictable stress.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0cec7eb2-cb95-5751-a161-3d2a4033f22b","slug":"mouse-grm2","display_name":"Mouse metabotropic glutamate receptor 2 / Grm2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"964f7af1-e158-5cf7-9874-a9be7501ced7","slug":"mouse-alc-stress-behavior","display_name":"Mouse stress-model behavioral response to acetylcarnitine","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse knockout and stress-behavior experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Behavioral tests are not human depression outcomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Removing the receptor tested whether the rodent effect depended on it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"L-acetylcarnitine causes rapid antidepressant effects through the epigenetic induction of mGlu2 receptors. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23382250/ · DOI 10.1073/pnas.1216100110","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"47a0fb46-207c-5f37-a4a0-d6c2858b82e9","evidence_kind":"source_excerpt","locator":"Lines 322-328","start_line":322,"end_line":328,"excerpt":"## l-carnitine-brain-receptor-required\nRemoving the receptor tested whether the rodent effect depended on it.\nAcetyl-L-carnitine did not produce the reported behavioral benefit in mGlu2-knockout mice exposed to chronic unpredictable stress.\nModel: Mouse knockout and stress-behavior experiments.\nLimitations: Behavioral tests are not human depression outcomes.\nEvidence access: Primary abstract\nL-acetylcarnitine causes rapid antidepressant effects through the epigenetic induction of mGlu2 receptors. · 2013 · https://pubmed.ncbi.nlm.nih.gov/23382250/ · DOI 10.1073/pnas.1216100110","model_system":"Mouse knockout and stress-behavior experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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