{"id":"6d32a1c0-ed99-5761-8db3-72efec72fa67","stable_key":"53d9b98d-e3a0-5416-bc88-adbe42a8cb1b:curcumin-bcrp-inhibition","predicate":"inhibits","statement":"Curcumin inhibited human BCRP-mediated sulfasalazine transport in vesicles, Ki 0.70 +/- 0.41 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"882bfbc2-b9d5-549e-9289-ddbbcba80205","mechanism_event_label":"A drug-export transporter can be inhibited in the test system.","subject":{"id":"0e625439-d093-5d1b-8713-dcfdafcf6811","slug":"curcumin","display_name":"Curcumin","entity_type_key":"small_molecule"},"object":{"id":"a52e10c2-fd56-564b-8c05-27e9fce27cd6","slug":"abcg2","display_name":"Human ABCG2 / breast cancer resistance protein","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"882bfbc2-b9d5-549e-9289-ddbbcba80205","stable_key":"53d9b98d-e3a0-5416-bc88-adbe42a8cb1b:curcumin-bcrp-inhibition-event","event_type":"biochemical_relationship","label":"A drug-export transporter can be inhibited in the test system.","description":"Curcumin inhibited human BCRP-mediated sulfasalazine transport in vesicles, Ki 0.70 +/- 0.41 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a5eafc6b-a85d-555d-9a24-13748e96218f","slug":"sulfasalazine","display_name":"Sulfasalazine","entity_type_key":"small_molecule"},"role":"transported_drug","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0e625439-d093-5d1b-8713-dcfdafcf6811","slug":"curcumin","display_name":"Curcumin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a52e10c2-fd56-564b-8c05-27e9fce27cd6","slug":"abcg2","display_name":"Human ABCG2 / breast cancer resistance protein","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/curcumin-research/22300367.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a8ec64e7dff4a52838fa067fdc9418ee3ebe768c4a4d53629bd84f9f0ffe67fe\", \"start_char\": 0, \"end_char\": 1713, \"text_sha256\": \"a8ec64e7dff4a52838fa067fdc9418ee3ebe768c4a4d53629bd84f9f0ffe67fe\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human pharmacokinetics, transporter vesicles and mouse knockout experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Human: curcumin 2 g before sulfasalazine 100 micrograms or 2 g; eight participants. 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Transporter inference is supported by separate assays, not a universal effect on every BCRP substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Curcumin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"curcumin","display_name":"Curcumin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human transporter/human participants; mouse claims separately scoped","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A drug-export transporter can be inhibited in the test system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[curcumin-p22300367] Pharmacokinetic interaction study of sulphasalazine in healthy subjects and the impact of curcumin as an in vivo inhibitor of BCRP. (2012). https://pubmed.ncbi.nlm.nih.gov/22300367/ DOI: 10.1111/j.1476-5381.2012.01887.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal drug transport and plasma","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f289cd14-b0af-5348-a234-74a5358dd438","evidence_kind":"source_excerpt","locator":"Lines 775-786","start_line":775,"end_line":786,"excerpt":"### curcumin-bcrp-inhibition\nCurcumin inhibited human BCRP-mediated sulfasalazine transport in vesicles, Ki 0.70 +/- 0.41 micromolar.\nCondition category: normal\nnutrient_topic: Curcumin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A drug-export transporter can be inhibited in the test system.\norganism: Human transporter/human participants; mouse claims separately scoped\ntissue_or_cell_type: Intestinal drug transport and plasma\nexperimental_model: Human pharmacokinetics, transporter vesicles and mouse knockout experiment\nlimitations: Small dose- and formulation-specific drug study. Transporter inference is supported by separate assays, not a universal effect on every BCRP substrate.\nexposure: Human: curcumin 2 g before sulfasalazine 100 micrograms or 2 g; eight participants. Mouse: 300-400 mg/kg curcumin\nevidence_span: {\"source_cache\": \"artifacts/curcumin-research/22300367.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"a8ec64e7dff4a52838fa067fdc9418ee3ebe768c4a4d53629bd84f9f0ffe67fe\", \"start_char\": 0, \"end_char\": 1713, \"text_sha256\": \"a8ec64e7dff4a52838fa067fdc9418ee3ebe768c4a4d53629bd84f9f0ffe67fe\"}\n[curcumin-p22300367] Pharmacokinetic interaction study of sulphasalazine in healthy subjects and the impact of curcumin as an in vivo inhibitor of BCRP. (2012). https://pubmed.ncbi.nlm.nih.gov/22300367/ DOI: 10.1111/j.1476-5381.2012.01887.x","model_system":"Human pharmacokinetics, transporter vesicles and mouse knockout experiment","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [curcumin-p22300367] Pharmacokinetic interaction study of sulphasalazine in healthy subjects and the impact of curcumin as an in vivo inhibitor of BCRP. (2012). https://pubmed.ncbi.nlm.nih.gov/22300367/ DOI: 10.1111/j.1476-5381.2012.01887.x","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"11d29ea9-3af1-5404-a68d-6f129e23edee","stable_key":"import-53d9b98d-e3a0-5416-bc88-adbe42a8cb1b","title":"Curcumin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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