{"id":"6cfb17a5-ba29-5885-ac9f-0608bfccbbca","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-cd40-ifng-ido","predicate":"potentiates_ifng_induction","statement":"CD40 ligand increased IFN-gamma-driven tryptophan catabolism in human monocyte-derived macrophages.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f1d2d518-de24-5f5b-9bbb-d20ff0066201","mechanism_event_label":"Two immune signals jointly increased local consumption.","subject":{"id":"dadfe958-b098-5edd-8dd4-4c9d190a9016","slug":"cd40lg","display_name":"Human CD40 ligand / CD40LG","entity_type_key":"protein"},"object":{"id":"9edba4aa-c2f5-5a4e-9750-15e9a38b1822","slug":"ido1","display_name":"Human indoleamine 2,3-dioxygenase 1 / IDO1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f1d2d518-de24-5f5b-9bbb-d20ff0066201","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-cd40-ifng-ido-event","event_type":"observed_relationship","label":"Two immune signals jointly increased local consumption.","description":"CD40 ligand increased IFN-gamma-driven tryptophan catabolism in human monocyte-derived macrophages.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dadfe958-b098-5edd-8dd4-4c9d190a9016","slug":"cd40lg","display_name":"Human CD40 ligand / CD40LG","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9edba4aa-c2f5-5a4e-9750-15e9a38b1822","slug":"ido1","display_name":"Human indoleamine 2,3-dioxygenase 1 / IDO1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"393e7fb8-8b09-5b6a-b7e4-a31a78b4fcfb","slug":"ifng","display_name":"Human interferon gamma / IFNG","entity_type_key":"cytokine"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, Figure 5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human macrophage and activated-T-cell coculture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that systemic tryptophan intake determines every immune response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Two immune signals jointly increased local consumption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of T cell proliferation by macrophage tryptophan catabolism. · 1999 · https://pubmed.ncbi.nlm.nih.gov/10224276/ · DOI 10.1084/jem.189.9.1363","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"549a8bd9-f0ad-5e19-be52-c9379d587f4f","evidence_kind":"source_excerpt","locator":"Lines 346-352","start_line":346,"end_line":352,"excerpt":"## tryptophan-cd40-ifng-ido\nTwo immune signals jointly increased local consumption.\nCD40 ligand increased IFN-gamma-driven tryptophan catabolism in human monocyte-derived macrophages.\nModel: Human macrophage and activated-T-cell coculture.\nLimitations: Not evidence that systemic tryptophan intake determines every immune response.\nEvidence access: Primary full text, Figure 5\nInhibition of T cell proliferation by macrophage tryptophan catabolism. · 1999 · https://pubmed.ncbi.nlm.nih.gov/10224276/ · DOI 10.1084/jem.189.9.1363","model_system":"Human macrophage and activated-T-cell coculture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}