{"id":"6cc4b7af-251e-5422-bdff-5880c7e9efc9","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-ogdh-human-cofactor-structure","predicate":"binds","statement":"The human OGDH cryo-EM model 7WGR contains Mg and ThDP as well as a separate Ca-binding site.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ce389172-c6c8-5f8f-bc93-13e67ee4444b","mechanism_event_label":"The human alpha-ketoglutarate-processing enzyme has distinct cofactor and calcium-regulatory architecture.","subject":{"id":"2fb34fcb-d648-52c6-ae1b-308d2b7acd05","slug":"ogdh","display_name":"Human 2-oxoglutarate dehydrogenase E1","entity_type_key":"protein"},"object":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"ce389172-c6c8-5f8f-bc93-13e67ee4444b","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-ogdh-human-cofactor-structure-event","event_type":"biochemical_relationship","label":"The human alpha-ketoglutarate-processing enzyme has distinct cofactor and calcium-regulatory architecture.","description":"The human OGDH cryo-EM model 7WGR contains Mg and ThDP as well as a separate Ca-binding site.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"role":"bound cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"distinct structural site","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2fb34fcb-d648-52c6-ae1b-308d2b7acd05","slug":"ogdh","display_name":"Human 2-oxoglutarate dehydrogenase E1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Magnesium and thiamine-derived ThDP intersect at mitochondrial carbon metabolism; purified-enzyme responses do not measure whole-body energy supply.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human OGDH cryo-EM structure; deposited coordinate model 7WGR.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structure does not establish a clinical Mg threshold or metal replacement equivalence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The human alpha-ketoglutarate-processing enzyme has distinct cofactor and calcium-regulatory architecture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zhong-2022-ogdh] Structural basis for the activity and regulation of human α-ketoglutarate dehydrogenase revealed by Cryo-EM (2022). https://pubmed.ncbi.nlm.nih.gov/35272141/ DOI: 10.1016/j.bbrc.2022.02.093","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"structure_url","value_text":"https://pdbj.org/mine/summary/7wgr","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Recombinant mitochondrial OGDH","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"34ed8db1-44a8-5084-8564-df2cf11ebef6","evidence_kind":"source_excerpt","locator":"Lines 662-673","start_line":662,"end_line":673,"excerpt":"### mg-ogdh-human-cofactor-structure\nThe human OGDH cryo-EM model 7WGR contains Mg and ThDP as well as a separate Ca-binding site.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The human alpha-ketoglutarate-processing enzyme has distinct cofactor and calcium-regulatory architecture.\norganism: Homo sapiens\ntissue_or_cell_type: Recombinant mitochondrial OGDH\nexperimental_model: Human OGDH cryo-EM structure; deposited coordinate model 7WGR.\nlimitations: Structure does not establish a clinical Mg threshold or metal replacement equivalence.\ncross_nutrient: Magnesium and thiamine-derived ThDP intersect at mitochondrial carbon metabolism; purified-enzyme responses do not measure whole-body energy supply.\nstructure_url: https://pdbj.org/mine/summary/7wgr\n[zhong-2022-ogdh] Structural basis for the activity and regulation of human α-ketoglutarate dehydrogenase revealed by Cryo-EM (2022). https://pubmed.ncbi.nlm.nih.gov/35272141/ DOI: 10.1016/j.bbrc.2022.02.093","model_system":"Human OGDH cryo-EM structure; deposited coordinate model 7WGR.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zhong-2022-ogdh] Structural basis for the activity and regulation of human α-ketoglutarate dehydrogenase revealed by Cryo-EM (2022). https://pubmed.ncbi.nlm.nih.gov/35272141/ DOI: 10.1016/j.bbrc.2022.02.093","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}