{"id":"6c5c8e5d-9bb8-53ab-ae53-31db402eff7d","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:alveolar-nfkb","predicate":"decreases_in_recorded_experiment","statement":"Thymulin reduced NF-kappa-B nuclear signaling in LPS-challenged fetal rat alveolar type II cells.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d8b3e1f8-1513-5ca7-8e6e-d619fff68625","mechanism_event_label":"Thymulin reduced NF-kappa-B nuclear signaling in LPS-challenged fetal rat alveolar type II cells.","subject":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"object":{"id":"59a1b15a-6a5d-5946-83f2-a6c48355b5fe","slug":"rat-nfkb-nuclear-signaling","display_name":"Rat NF-kappa-B nuclear signaling","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d8b3e1f8-1513-5ca7-8e6e-d619fff68625","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:alveolar-nfkb-event","event_type":"experimental_observation","label":"Thymulin reduced NF-kappa-B nuclear signaling in LPS-challenged fetal rat alveolar type II cells.","description":"| Fetal rat alveolar type II cells challenged with LPS | Thymulin increased cAMP, reduced NF-κB nuclear signaling and reduced IL-1β release; IL-6 and TNF-α were not similarly suppressed. | Zinc alone also changed cytokine outputs. cAMP-associated effects do not identify a GPCR or establish sole mediation. This paper is not the retracted hippocampal paper discussed below. [2009 experiment](https://pubmed.ncbi.nlm.nih.gov/19850345/) |","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"tested factor","stoichiometry":null,"state_label":"Thymulin plus LPS","sequence_order":0,"notes":""},{"entity":{"id":"59a1b15a-6a5d-5946-83f2-a6c48355b5fe","slug":"rat-nfkb-nuclear-signaling","display_name":"Rat NF-kappa-B nuclear signaling","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"separate control exposure","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary bibliographic record and abstract checked; full methods/figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"peptide exposure","comparator":"LPS-matched control","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Thymulin plus LPS\", \"comparator\": \"LPS-matched control\", \"endpoint\": \"Thymulin reduced NF-kappa-B nuclear signaling in LPS-challenged fetal rat alveolar type II cells.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"thymulin\", \"state\": \"peptide exposure\"}, {\"entity_slug\": null, \"state\": \"LPS challenge\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Fetal rat alveolar type II cells challenged with LPS; zinc-alone controls also affected cytokines. Distinct from the retracted hippocampal paper.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Thymulin reduced NF-kappa-B nuclear signaling in LPS-challenged fetal rat alveolar type II cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Thymulin and zinc (Zn2+)-mediated inhibition of endotoxin-induced production of proinflammatory cytokines and NF-kappaB nuclear translocation and activation in the alveolar epithelium: unraveling the molecular immunomodulatory, anti-inflammatory effect of thymulin/Zn2+ in vitro. | 2009 | DOI 10.1016/j.molimm.2009.09.034 | PMID 19850345 | https://pubmed.ncbi.nlm.nih.gov/19850345/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 69-69; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d57460d0-2c8d-59b0-a9d9-588485f58f4a","evidence_kind":"source_excerpt","locator":"Lines 69-69","start_line":69,"end_line":69,"excerpt":"| Fetal rat alveolar type II cells challenged with LPS | Thymulin increased cAMP, reduced NF-κB nuclear signaling and reduced IL-1β release; IL-6 and TNF-α were not similarly suppressed. | Zinc alone also changed cytokine outputs. cAMP-associated effects do not identify a GPCR or establish sole mediation. This paper is not the retracted hippocampal paper discussed below. [2009 experiment](https://pubmed.ncbi.nlm.nih.gov/19850345/) |","model_system":"Fetal rat alveolar type II cells challenged with LPS; zinc-alone controls also affected cytokines. Distinct from the retracted hippocampal paper.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e1871d49-9c6b-547b-a1bb-6e2b9270ca89","stable_key":"import-18100170-a014-51c3-adeb-9dab4162b8cc","title":"Thymulin: detailed mechanisms of action (reviewed 3 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"6e8ed0d7a5e23fc822f2ab418e0c4fb2411c01fcaa330039e8d7d93218cacc88","revision_id":"8c0e3449-9bbd-57c0-bb5e-de83f044f2ac","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}