{"id":"6be2de5c-f986-5545-be43-f762c26e2fe2","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-pparg-antagonism","predicate":"suppresses","statement":"Adding t10,c12, but not c9,t11 CLA, suppressed ligand-stimulated PPAR-gamma reporter activation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"89091748-589a-5bc1-8f78-0409c5e02595","mechanism_event_label":"An isomer could interfere with activation of the regulator.","subject":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"object":{"id":"331ba961-d73f-5ded-87bd-6b15585d3c4d","slug":"human-adipocyte-pparg-reporter","display_name":"Human adipocyte PPAR-gamma reporter activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"89091748-589a-5bc1-8f78-0409c5e02595","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-pparg-antagonism-event","event_type":"observed_relationship","label":"An isomer could interfere with activation of the regulator.","description":"Adding t10,c12, but not c9,t11 CLA, suppressed ligand-stimulated PPAR-gamma reporter activation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"331ba961-d73f-5ded-87bd-6b15585d3c4d","slug":"human-adipocyte-pparg-reporter","display_name":"Human adipocyte PPAR-gamma reporter activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a85c068b-9c0b-5a08-bad7-66ff3ef2af19","slug":"cla-cis9-trans11","display_name":"cis-9,trans-11 conjugated linoleic acid / rumenic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human adipocyte culture and reporter experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The 2003 transient-transfection study reported antagonism with both isomers; assay context remains relevant.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"conjugated-linoleic-acid","display_name":"Conjugated linoleic acid / CLA isomer family","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"An isomer could interfere with activation of the regulator.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Trans-10, cis-12 conjugated linoleic acid antagonizes ligand-dependent PPARgamma activity in primary cultures of human adipocytes. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18287349/ · DOI 10.1093/jn/138.3.455","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"13633411-39ea-5881-b429-04aa329b7e10","evidence_kind":"source_excerpt","locator":"Lines 102-108","start_line":102,"end_line":108,"excerpt":"## cla-pparg-antagonism\nAn isomer could interfere with activation of the regulator.\nAdding t10,c12, but not c9,t11 CLA, suppressed ligand-stimulated PPAR-gamma reporter activation.\nModel: Primary human adipocyte culture and reporter experiments.\nLimitations: The 2003 transient-transfection study reported antagonism with both isomers; assay context remains relevant.\nEvidence access: Primary abstract\nTrans-10, cis-12 conjugated linoleic acid antagonizes ligand-dependent PPARgamma activity in primary cultures of human adipocytes. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18287349/ · DOI 10.1093/jn/138.3.455","model_system":"Primary human adipocyte culture and reporter experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"45cfa6e3-2e7d-57c1-8c35-65302b7b71db","stable_key":"import-ee4227e5-80d7-55e9-ada7-3c5a16f93ac9","title":"Conjugated linoleic acid: isomers, signaling, nutrient interactions and discovery (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f9fe73a8a4008d52061c65abe6de7a9806da75f1c478c5adace9387b8ffaad9e","revision_id":"21196d9c-aaab-5fe1-8f32-2987c94289c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}