{"id":"6b94bbe7-7ee6-5cf1-9b80-a174bd2f01d5","stable_key":"3d41d18a-e13a-59a0-a081-55bfcdd43e55:ceylon-leaf-ache","predicate":"inhibits","statement":"The leaf oil inhibited the assay acetylcholinesterase preparation, with reported IC50 16.03 micrograms/mL.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0da572b6-fddc-554e-82e0-18ee4231ebd3","mechanism_event_label":"An enzyme assay found inhibition; this does not establish improved memory.","subject":{"id":"41349963-b67e-5285-a461-d4164fbab8b9","slug":"ceylon-leaf-oil-2023","display_name":"Ceylon leaf oil sample characterized in the 2023 enzyme study","entity_type_key":"chemical_species"},"object":{"id":"4dd9b69b-4a36-53cf-b70c-8ef7e9e298d7","slug":"ceylon-leaf-study-ache","display_name":"Acetylcholinesterase assay preparation in the 2023 leaf-oil study; species unresolved here","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"0da572b6-fddc-554e-82e0-18ee4231ebd3","stable_key":"3d41d18a-e13a-59a0-a081-55bfcdd43e55:ceylon-leaf-ache-event","event_type":"biochemical_relationship","label":"An enzyme assay found inhibition; this does not establish improved memory.","description":"The leaf oil inhibited the assay acetylcholinesterase preparation, with reported IC50 16.03 micrograms/mL.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"41349963-b67e-5285-a461-d4164fbab8b9","slug":"ceylon-leaf-oil-2023","display_name":"Ceylon leaf oil sample characterized in the 2023 enzyme study","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4dd9b69b-4a36-53cf-b70c-8ef7e9e298d7","slug":"ceylon-leaf-study-ache","display_name":"Acetylcholinesterase assay preparation in the 2023 leaf-oil study; species unresolved here","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ceylon-research/36676085.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c71e19c572310e80b103949f460ac437e9a564daae423e1de3f17964f2c92291\", \"start_char\": 0, \"end_char\": 1755, \"text_sha256\": \"c71e19c572310e80b103949f460ac437e9a564daae423e1de3f17964f2c92291\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Composition, chemical redox and cell-free enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"One leaf oil sample; concentration-response assays in micrograms/mL","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This leaf sample is not representative of all leaf oils. Chemical reduction is not mineral absorption; enzyme inhibition is not disease treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ceylon","display_name":"Ceylon cinnamon / Cinnamomum verum bark preparations","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"C. zeylanicum leaf sample; human CA II and other assay preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An enzyme assay found inhibition; this does not establish improved memory.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ceylon-p36676085] Comprehensive Metabolite Profiling of Cinnamon (Cinnamomum zeylanicum) Leaf Oil Using LC-HR/MS, GC/MS, and GC-FID: Determination of Antiglaucoma, Antioxidant, Anticholinergic, and Antidiabetic Profiles. (2023). https://pubmed.ncbi.nlm.nih.gov/36676085/ DOI: 10.3390/life13010136","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Leaf essential oil and purified-enzyme assays","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c5cdc2de-3ab5-5d22-ba9e-14e033686ee4","evidence_kind":"source_excerpt","locator":"Lines 142-153","start_line":142,"end_line":153,"excerpt":"### ceylon-leaf-ache\nThe leaf oil inhibited the assay acetylcholinesterase preparation, with reported IC50 16.03 micrograms/mL.\nCondition category: normal\nnutrient_topic: Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An enzyme assay found inhibition; this does not establish improved memory.\norganism: C. zeylanicum leaf sample; human CA II and other assay preparations\ntissue_or_cell_type: Leaf essential oil and purified-enzyme assays\nexperimental_model: Composition, chemical redox and cell-free enzyme assays\nlimitations: This leaf sample is not representative of all leaf oils. Chemical reduction is not mineral absorption; enzyme inhibition is not disease treatment.\nexposure: One leaf oil sample; concentration-response assays in micrograms/mL\nevidence_span: {\"source_cache\": \"artifacts/ceylon-research/36676085.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c71e19c572310e80b103949f460ac437e9a564daae423e1de3f17964f2c92291\", \"start_char\": 0, \"end_char\": 1755, \"text_sha256\": \"c71e19c572310e80b103949f460ac437e9a564daae423e1de3f17964f2c92291\"}\n[ceylon-p36676085] Comprehensive Metabolite Profiling of Cinnamon (Cinnamomum zeylanicum) Leaf Oil Using LC-HR/MS, GC/MS, and GC-FID: Determination of Antiglaucoma, Antioxidant, Anticholinergic, and Antidiabetic Profiles. 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