{"id":"6b84bdf8-2756-5817-a807-adb76073ef12","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:dhq-dhs","predicate":"converted_to_in_recorded_reaction","statement":"AroD dehydrates 3-dehydroquinate to 3-dehydroshikimate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"87be636e-8676-5e57-ab4d-56ccc014fdc1","mechanism_event_label":"AroD dehydrates 3-dehydroquinate to 3-dehydroshikimate.","subject":{"id":"1884ebad-8172-5332-8c57-d4f7fe73a008","slug":"3-dehydroquinate","display_name":"3-Dehydroquinate / DHQ","entity_type_key":"small_molecule"},"object":{"id":"320d1df4-8da0-5e1a-bb17-5140e264bf06","slug":"3-dehydroshikimate","display_name":"3-Dehydroshikimate / DHS","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"87be636e-8676-5e57-ab4d-56ccc014fdc1","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:dhq-dhs-event","event_type":"biochemical_reaction","label":"AroD dehydrates 3-dehydroquinate to 3-dehydroshikimate.","description":"**The two steps before shikimate.** E. coli AroB converts DAHP to 3-dehydroquinate (DHQ); AroD dehydrates DHQ to 3-dehydroshikimate (DHS). DHS and shikimate are different metabolites. Separating these intermediates matters when metabolomics shows them accumulating during downstream inhibition. [Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate.](https://pubmed.ncbi.nlm.nih.gov/31937786/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1884ebad-8172-5332-8c57-d4f7fe73a008","slug":"3-dehydroquinate","display_name":"3-Dehydroquinate / DHQ","entity_type_key":"small_molecule"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"320d1df4-8da0-5e1a-bb17-5140e264bf06","slug":"3-dehydroshikimate","display_name":"3-Dehydroshikimate / DHS","entity_type_key":"small_molecule"},"role":"output","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"95a6161f-6715-5894-9024-dd35d2b3f9fe","slug":"ecoli-arod","display_name":"Escherichia coli 3-dehydroquinate dehydratase / AroD","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. 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No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"AroD dehydrates 3-dehydroquinate to 3-dehydroshikimate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate. | 2020 | DOI 10.1038/s41467-019-14024-1 | PMID 31937786 | https://pubmed.ncbi.nlm.nih.gov/31937786/ | https://doi.org/10.1038/s41467-019-14024-1 | https://pmc.ncbi.nlm.nih.gov/articles/PMC6959354/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 11-11; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d2044c45-2e97-558e-8621-7d21a78895a4","evidence_kind":"source_excerpt","locator":"Lines 11-11","start_line":11,"end_line":11,"excerpt":"**The two steps before shikimate.** E. coli AroB converts DAHP to 3-dehydroquinate (DHQ); AroD dehydrates DHQ to 3-dehydroshikimate (DHS). DHS and shikimate are different metabolites. Separating these intermediates matters when metabolomics shows them accumulating during downstream inhibition. [Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate.](https://pubmed.ncbi.nlm.nih.gov/31937786/)","model_system":"Escherichia coli biochemical pathway; source-specific enzyme evidence recorded in the passage.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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