{"id":"6b6a41fd-486f-5168-b49d-cfb726c7e94f","stable_key":"1bbf9d44-8d2b-5b35-8f82-f6876558b0b3:cyclosporine-inhibits-permeability-transition","predicate":"inhibits","statement":"Cyclosporin A inhibited the large-amplitude swelling that isolated rat liver and heart mitochondria show when exposed to 250 micromolar calcium, with the number of cyclosporin-binding sites titrated at 113.7 plus or minus 5.0 and 124.3 plus or minus 11.2 picomoles per milligram of protein and a Ki of about 5 nanomolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a28de503-af07-5723-829f-16035a39296d","mechanism_event_label":"Cyclosporin A inhibited the large-amplitude swelling that isolated rat liver and heart mitochondria show when exposed to 250 micromolar calcium, with the number of cyclosporin-binding sites titrated a","subject":{"id":"b199063a-fae6-5c5c-9cb4-22c10453bbac","slug":"cyclosporine","display_name":"Cyclosporine","entity_type_key":"drug"},"object":{"id":"bcb87e8d-5a43-5c26-926a-ffc24ae61493","slug":"mitochondrial-permeability-transition","display_name":"Mitochondrial permeability transition","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a28de503-af07-5723-829f-16035a39296d","stable_key":"1bbf9d44-8d2b-5b35-8f82-f6876558b0b3:cyclosporine-inhibits-permeability-transition-event","event_type":"biochemical_relationship","label":"Cyclosporin A inhibited the large-amplitude swelling that isolated rat liver and heart mitochondria show when exposed to 250 micromolar calcium, with the number of cyclosporin-binding sites titrated a","description":"Calcium-induced inner-membrane pore opening","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b199063a-fae6-5c5c-9cb4-22c10453bbac","slug":"cyclosporine","display_name":"Cyclosporine","entity_type_key":"drug"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bcb87e8d-5a43-5c26-926a-ffc24ae61493","slug":"mitochondrial-permeability-transition","display_name":"Mitochondrial permeability transition","entity_type_key":"cellular_process"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. 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The proposed interaction between the two is the authors' model, not a measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rat liver and heart mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cyclosporin A inhibited the large-amplitude swelling that isolated rat liver and heart mitochondria show when exposed to 250 micromolar calcium, with the number of cyclosporin-binding sites titrated at 113.7 plus or minus 5.0 and 124.3 plus or minus 11.2 picomoles per milligram of protein and a Ki of about 5 nanomolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of Ca2+-induced large-amplitude swelling of liver and heart mitochondria by cyclosporin is probably caused by the inhibitor binding to mitochondrial-matrix peptidyl-prolyl cis-trans isomerase and preventing it interacting with the adenine nucleotide translocase. 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No reference carries a recorded retraction, erratum or expression of concern. Each of the seven is a separate laboratory and each carries its own lineage key, so none of them can be counted twice as independent support. Study-specific concentrations, kinetic constants and limitations retained. Not publisher full text.","file_path":"","sha256":"499d00555a629cba2271c3888d7e93627949891d565c4c78c843610de2913080","revision_id":"263ee68a-4184-51d0-a977-197d7ddb7a4d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}