{"id":"6b224eb1-a868-5a57-845f-f5897ee11ed9","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-rara-rxra-cooperative-binding","predicate":"heterodimerizes-with","statement":"RXRA associated with RARA and cooperatively bound retinoid response DNA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d0b0b9e1-9118-50ae-ad49-8d0715ec1ace","mechanism_event_label":"Two distinct receptor proteins assemble a DNA-binding complex.","subject":{"id":"7dc1ce11-f208-5187-95d9-96b07927df6a","slug":"rxra","display_name":"Retinoid X receptor alpha","entity_type_key":"protein"},"object":{"id":"231bc87f-843e-57b3-ad84-06f63f656383","slug":"rara","display_name":"Retinoic acid receptor alpha","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d0b0b9e1-9118-50ae-ad49-8d0715ec1ace","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-rara-rxra-cooperative-binding-event","event_type":"biochemical_relationship","label":"Two distinct receptor proteins assemble a DNA-binding complex.","description":"RXRA associated with RARA and cooperatively bound retinoid response DNA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ce392c9e-6483-5914-b18b-d6a2520dbbf6","slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"},"role":"nutrient-context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5b81b3c4-fadc-54c2-bb8f-107f42bdaa33","slug":"rara-rxra","display_name":"RAR alpha-RXR alpha heterodimer","entity_type_key":"protein_complex"},"role":"complex","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7dc1ce11-f208-5187-95d9-96b07927df6a","slug":"rxra","display_name":"Retinoid X receptor alpha","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"231bc87f-843e-57b3-ad84-06f63f656383","slug":"rara","display_name":"Retinoic acid receptor alpha","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_locator","value_text":"Figure 2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant receptors, gel shifts, coimmunoprecipitation and CV-1 reporters.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Receptor coexpression/reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Synthetic response elements; 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topical membership is not evidence of a direct dietary effect.\nplain_language: Two distinct receptor proteins assemble a DNA-binding complex.\norganism: Recombinant receptors\ntissue_or_cell_type: Cell-free DNA-binding assay\nexperimental_model: Recombinant receptors, gel shifts, coimmunoprecipitation and CV-1 reporters.\nlimitations: Synthetic response elements; not every genomic site.\nevidence_locator: Figure 2\nexposure: Receptor coexpression/reconstitution\n[va-kliewer-1992] Retinoid X receptor interacts with nuclear receptors in retinoic acid, thyroid hormone and vitamin D3 signalling (1992). https://pubmed.ncbi.nlm.nih.gov/1310351/ DOI: 10.1038/355446a0","model_system":"Recombinant receptors, gel shifts, coimmunoprecipitation and CV-1 reporters.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [va-kliewer-1992] Retinoid X receptor interacts with nuclear receptors in retinoic acid, thyroid hormone and vitamin D3 signalling (1992). https://pubmed.ncbi.nlm.nih.gov/1310351/ DOI: 10.1038/355446a0","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0c5474e2-be48-547b-b287-9c07a59c9ff1","stable_key":"import-dff9f743-3766-5f20-a71e-d6d2bd5bfb6f","title":"Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b217224b262abbd77d6a0c8c9beefff9c022fae4d31e2a5273915d769b9a6546","revision_id":"61008909-871f-575c-b5e2-21542689ea3c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}