{"id":"6b13bfe8-90ed-593d-b5a6-72b223f364b7","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-rela-knockdown","predicate":"knockdown_attenuates","statement":"p65 siRNA depletion attenuated CLA-induced loss of GLUT4 and PPAR-gamma proteins and suppression of glucose uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0cc59ab4-c913-548f-99ad-8c7f1b4d375e","mechanism_event_label":"Removing a signaling component weakened the adverse response.","subject":{"id":"ecf9653e-8710-540d-953a-78ef96bf4752","slug":"rela","display_name":"Human NF-kappa-B p65 / RELA","entity_type_key":"protein"},"object":{"id":"65045ae0-4ce9-5f15-abb1-62dab932f963","slug":"human-adipocyte-glucose-uptake","display_name":"Human adipocyte insulin-stimulated glucose uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0cc59ab4-c913-548f-99ad-8c7f1b4d375e","stable_key":"ee4227e5-80d7-55e9-ada7-3c5a16f93ac9:cla-rela-knockdown-event","event_type":"observed_relationship","label":"Removing a signaling component weakened the adverse response.","description":"p65 siRNA depletion attenuated CLA-induced loss of GLUT4 and PPAR-gamma proteins and suppression of glucose uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ecf9653e-8710-540d-953a-78ef96bf4752","slug":"rela","display_name":"Human NF-kappa-B p65 / RELA","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"65045ae0-4ce9-5f15-abb1-62dab932f963","slug":"human-adipocyte-glucose-uptake","display_name":"Human adipocyte insulin-stimulated glucose uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7f456e18-fabc-532d-bbfe-cd3a8cf1b348","slug":"pparg","display_name":"Human PPAR gamma / PPARG","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"0b58e90f-c976-5f65-82e5-8735455b9094","slug":"slc2a4","display_name":"Human glucose transporter 4 / SLC2A4","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4405f918-c17d-5365-b873-1678989f8ac1","slug":"cla-trans10-cis12","display_name":"trans-10,cis-12 conjugated linoleic acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human adipocyte RNA-interference experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Supports a contribution in this model, not universal necessity in all tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"CLA collection; 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