{"id":"6abf2fc4-f222-5414-8ff5-1b75a413a294","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-ketone-synaptic-response","predicate":"reduces_tested","statement":"Mouse hippocampal slices exposed to 10 mM lithium acetoacetate had lower miniature excitatory-current amplitude and frequency than lithium-chloride controls.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f2285376-d858-5a10-9372-079e3ebbfc78","mechanism_event_label":"A controlled slice experiment connected the transport finding with synaptic output.","subject":{"id":"1b77cb4a-1f79-50e2-b4f0-fd0953cb914e","slug":"acetoacetate","display_name":"Acetoacetate","entity_type_key":"small_molecule"},"object":{"id":"0ecd6985-dc3a-5c75-9e97-4c7ae7054060","slug":"mouse-acetoacetate-hippocampal-mepsc","display_name":"Mouse hippocampal miniature excitatory postsynaptic currents","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f2285376-d858-5a10-9372-079e3ebbfc78","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-ketone-synaptic-response-event","event_type":"observed_relationship","label":"A controlled slice experiment connected the transport finding with synaptic output.","description":"Mouse hippocampal slices exposed to 10 mM lithium acetoacetate had lower miniature excitatory-current amplitude and frequency than lithium-chloride controls.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1b77cb4a-1f79-50e2-b4f0-fd0953cb914e","slug":"acetoacetate","display_name":"Acetoacetate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0ecd6985-dc3a-5c75-9e97-4c7ae7054060","slug":"mouse-acetoacetate-hippocampal-mepsc","display_name":"Mouse hippocampal miniature excitatory postsynaptic currents","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse acute slices incubated for more than two hours; lithium matched between groups.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High bath exposure and slice conditions are explicit; do not substitute an oral ketone dose or attribute the difference to unmatched lithium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A controlled slice experiment connected the transport finding with synaptic output.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic control of vesicular glutamate transport and release. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20920794/ · DOI 10.1016/j.neuron.2010.09.002","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bbb5a6f7-addf-5ea7-8e02-992e1a6ae36e","evidence_kind":"source_excerpt","locator":"Lines 298-304","start_line":298,"end_line":304,"excerpt":"## glutamate-ketone-synaptic-response\nA controlled slice experiment connected the transport finding with synaptic output.\nMouse hippocampal slices exposed to 10 mM lithium acetoacetate had lower miniature excitatory-current amplitude and frequency than lithium-chloride controls.\nModel: Mouse acute slices incubated for more than two hours; lithium matched between groups.\nLimitations: High bath exposure and slice conditions are explicit; do not substitute an oral ketone dose or attribute the difference to unmatched lithium.\nEvidence access: Primary full text\nMetabolic control of vesicular glutamate transport and release. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20920794/ · DOI 10.1016/j.neuron.2010.09.002","model_system":"Mouse acute slices incubated for more than two hours; lithium matched between groups.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3da83bd75fddeb96d17af72d7e2ae04344779957650f266521f6c8ebd59fb54f","revision_id":"55196c72-94e4-540a-a869-81d35647b04a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}