{"id":"6aa93fc3-bf3d-540b-a0de-559983df7853","stable_key":"research:rd-lysosomal-selenop-recovery-requires-scly","predicate":"supports-selenop-derived-synthesis","statement":"RD cells used a lysosomal SELENOP recovery route dependent on SCLY.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"a7c329e9-d82d-5239-8a81-cdd95c5abe01","mechanism_event_label":"These rhabdomyosarcoma cells required SCLY for SELENOP selenium recovery.","subject":{"id":"7c1bfe9f-559b-598e-9cca-8d0b032919fd","slug":"scly","display_name":"SCLY","entity_type_key":"protein"},"object":{"id":"f4969f01-dda8-5b68-8352-07a92a6cfb50","slug":"selenoprotein-biosynthesis","display_name":"Selenoprotein biosynthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a7c329e9-d82d-5239-8a81-cdd95c5abe01","stable_key":"research:rd-lysosomal-selenop-recovery-requires-scly","event_type":"processing-dependence","label":"These rhabdomyosarcoma cells required SCLY for SELENOP selenium recovery.","description":"RD cells used a lysosomal SELENOP recovery route dependent on SCLY.","status":"active","compartment":null,"participants":[{"entity":{"id":"7c1bfe9f-559b-598e-9cca-8d0b032919fd","slug":"scly","display_name":"SCLY","entity_type_key":"protein"},"role":"required-enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c7fbd153-6444-50b2-a8be-6936e352a11c","slug":"selenop","display_name":"SELENOP","entity_type_key":"protein"},"role":"cargo","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f6588082-b338-5d8e-8776-c0eddeed7234","slug":"seleno-p-lysosomal-processing","display_name":"Lysosomal processing of SELENOP","entity_type_key":"cellular_process"},"role":"processing-context","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f4969f01-dda8-5b68-8352-07a92a6cfb50","slug":"selenoprotein-biosynthesis","display_name":"Selenoprotein biosynthesis","entity_type_key":"cellular_process"},"role":"readout","stoichiometry":null,"state_label":"SELENOP-derived selenium","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"RD rhabdomyosarcoma","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Perturbation assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Dependency does not locate SCLY catalysis inside lysosomes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"00748295-96b5-524d-982d-031a5a9eb225","evidence_kind":"curated_literature_summary","locator":"lines 570-580","start_line":570,"end_line":580,"excerpt":"## rd-lysosomal-selenop-recovery-requires-scly\n\nThese rhabdomyosarcoma cells required SCLY for SELENOP selenium recovery.\n\nRD cells used a lysosomal SELENOP recovery route dependent on SCLY.\n\nOrganism: human\nCell type: RD rhabdomyosarcoma\nExperimental model: Perturbation assays\nLimitations: Dependency does not locate SCLY catalysis inside lysosomes.\nPrimary reference: [An efficient selenium transport pathway of selenoprotein P utilizing a high-affinity ApoER2 receptor variant and being independent of selenocysteine lyase](https://pubmed.ncbi.nlm.nih.gov/37406814/)","model_system":"Perturbation assays","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"selenium-recycling","plain_language":"These rhabdomyosarcoma cells required SCLY for SELENOP selenium recovery.","evidence_scope":"direct_experimental","papers":[{"key":"mizuno-2023-lrp8-routing","title":"An efficient selenium transport pathway of selenoprotein P utilizing a high-affinity ApoER2 receptor variant and being independent of selenocysteine lyase","url":"https://pubmed.ncbi.nlm.nih.gov/37406814/","doi":"10.1016/j.jbc.2023.105009","year":2023,"model":"Human Jurkat and RD cells","summary":"Receptor variants and vesicle processing differ between cell lines; SCLY dependence is not universal."}]}}