{"id":"6a9b98e2-f8d0-521f-9be7-777023338e73","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-brain-thtp-from-thdp-and-pi","predicate":"converted-to","statement":"Rat brain mitochondria formed ThTP from ThDP and inorganic phosphate with respiratory substrates; ATP hydrolysis did not energize synthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"17612a4f-8ce8-5007-8676-7c2e8091427e","mechanism_event_label":"A third phosphate can be added to B1 using respiratory energy in this preparation.","subject":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"object":{"id":"baf0fa85-b008-55a1-b502-5adedf0382b8","slug":"thiamine-triphosphate","display_name":"Thiamine triphosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"17612a4f-8ce8-5007-8676-7c2e8091427e","stable_key":"46d15d9e-d3b5-544d-ba01-b785aa3e4f42:b1-brain-thtp-from-thdp-and-pi-event","event_type":"biochemical_relationship","label":"A third phosphate can be added to B1 using respiratory energy in this preparation.","description":"Rat brain mitochondria formed ThTP from ThDP and inorganic phosphate with respiratory substrates; 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coupled to respiratory energy","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Brain mitochondria","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"da4a404c-a836-555f-ab48-4ef2f6fdcaef","evidence_kind":"source_excerpt","locator":"Lines 602-615","start_line":602,"end_line":615,"excerpt":"### b1-brain-thtp-from-thdp-and-pi\nRat brain mitochondria formed ThTP from ThDP and inorganic phosphate with respiratory substrates; ATP hydrolysis did not energize synthesis.\nCondition category: normal\nnutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A third phosphate can be added to B1 using respiratory energy in this preparation.\norganism: Rattus norvegicus\ntissue_or_cell_type: Brain mitochondria\nexperimental_model: Isolated rat brain mitochondria; ThDP/Pi incubation, respiratory substrates and inhibitors.\nlimitations: Not a demonstrated human signaling function or a TPK1 reaction.\nreaction: ThDP + Pi <-> ThTP + water; coupled to respiratory energy\nevidence_spans: [{\"source_document\": \"artifacts/thiamine_transport_sources/gangolf-2010-thtp-source-record.json\", \"source_field\": \"resultList.result[0].abstractText\", \"start_char\": 0, \"end_char\": 1672}]\nevidence_locator: Results: ThTP synthesis in isolated mitochondria and energy coupling\nevidence-scope: Brain mitochondria\n[gangolf-2010-thtp] Thiamine Triphosphate Synthesis in Rat Brain Occurs in Mitochondria and Is Coupled to the Respiratory Chain (2010). https://pmc.ncbi.nlm.nih.gov/articles/PMC2804207/ DOI: 10.1074/jbc.M109.054379","model_system":"Isolated rat brain mitochondria; ThDP/Pi incubation, respiratory substrates and inhibitors.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [gangolf-2010-thtp] Thiamine Triphosphate Synthesis in Rat Brain Occurs in Mitochondria and Is Coupled to the Respiratory Chain (2010). https://pmc.ncbi.nlm.nih.gov/articles/PMC2804207/ DOI: 10.1074/jbc.M109.054379","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"158d2c03-ac8c-589f-8270-c468165ae346","stable_key":"import-46d15d9e-d3b5-544d-ba01-b785aa3e4f42","title":"Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f376512fb3310141315548015b387833e3af45146e73fb73cd02cee20e4ddb9c","revision_id":"53bc5eda-dec8-58cc-a56f-a9eb4ab036ef","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}