{"id":"6a8f3a0f-d427-57d7-a168-d51f13d34548","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-met-depletion-complex-i","predicate":"supports-complex-abundance","statement":"Riboflavin-free culture reduced complex I abundance; human 143B proteomics identified particularly strong loss of its NADH-oxidizing N-module subunits.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ec2b31cd-6c17-5efe-8b3b-f78c190cce45","mechanism_event_label":"Removing B2 reduced parts needed to build the respiratory enzyme.","subject":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"object":{"id":"f67ac698-de2e-55f5-8975-305eaadbb751","slug":"respiratory-complex-i","display_name":"Mitochondrial respiratory complex I","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"ec2b31cd-6c17-5efe-8b3b-f78c190cce45","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:b2-met-depletion-complex-i-event","event_type":"biochemical_relationship","label":"Removing B2 reduced parts needed to build the respiratory enzyme.","description":"Riboflavin-free culture reduced complex I abundance; 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unverified.","entity":null},{"dimension":"evidence_spans","value_text":"[{\"source_bundle\": \"artifacts/riboflavin_metabolism_sources.json\", \"source_key\": \"PMC10767280\", \"locator\": \"XML .//body//p\", \"paragraph_index\": 58, \"char_start\": 0, \"char_end\": 905, \"evidence_access\": \"full-text\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human 143B cells and mouse adult fibroblasts, riboflavin-free medium and separate DPI interventions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Riboflavin-free medium; matched control contained 1 micromolar riboflavin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture withdrawal; proteomic abundance does not establish a human blood threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens; Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Removing B2 reduced parts needed to build the respiratory enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[curtabbi-2024-fmn-assembly] Regulation of respiratory complex I assembly by FMN cofactor targeting (2024). https://pubmed.ncbi.nlm.nih.gov/38145589/ DOI: 10.1016/j.redox.2023.103001","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"143B cells and mouse adult fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"65495bb1-1c5f-5945-91a6-e65a758fb475","evidence_kind":"source_excerpt","locator":"Lines 609-620","start_line":609,"end_line":620,"excerpt":"### b2-met-depletion-complex-i\nRiboflavin-free culture reduced complex I abundance; human 143B proteomics identified particularly strong loss of its NADH-oxidizing N-module subunits.\nCondition category: nutrient_deficiency\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Removing B2 reduced parts needed to build the respiratory enzyme.\norganism: Homo sapiens; Mus musculus\ntissue_or_cell_type: 143B cells and mouse adult fibroblasts\nexperimental_model: Human 143B cells and mouse adult fibroblasts, riboflavin-free medium and separate DPI interventions.\nlimitations: Cell-culture withdrawal; proteomic abundance does not establish a human blood threshold.\nexposure: Riboflavin-free medium; matched control contained 1 micromolar riboflavin.\nevidence_spans: [{\"source_bundle\": \"artifacts/riboflavin_metabolism_sources.json\", \"source_key\": \"PMC10767280\", \"locator\": \"XML .//body//p\", \"paragraph_index\": 58, \"char_start\": 0, \"char_end\": 905, \"evidence_access\": \"full-text\"}]\n[curtabbi-2024-fmn-assembly] Regulation of respiratory complex I assembly by FMN cofactor targeting (2024). https://pubmed.ncbi.nlm.nih.gov/38145589/ DOI: 10.1016/j.redox.2023.103001","model_system":"Human 143B cells and mouse adult fibroblasts, riboflavin-free medium and separate DPI interventions.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [curtabbi-2024-fmn-assembly] Regulation of respiratory complex I assembly by FMN cofactor targeting (2024). https://pubmed.ncbi.nlm.nih.gov/38145589/ DOI: 10.1016/j.redox.2023.103001","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}