{"id":"6a053fb3-d662-5540-8ca3-8b281399a26a","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-stem-cell-vitamin-c","predicate":"reverses","statement":"Ascorbic-acid addition or proline withdrawal reversed the reported proline-induced stem-cell state and reduced the associated H3K9/H3K36 methylation pattern.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b7f80fe5-0d8c-5aca-85f3-d239b3b28afc","mechanism_event_label":"Vitamin C and proline intersect in this experimental epigenetic response.","subject":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"object":{"id":"0c7c34f5-86df-594c-b466-9dec4c6345c6","slug":"mouse-esc-proline-cell-state","display_name":"Proline-induced mouse embryonic-stem-cell state transition","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b7f80fe5-0d8c-5aca-85f3-d239b3b28afc","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-stem-cell-vitamin-c-event","event_type":"observed_relationship","label":"Vitamin C and proline intersect in this experimental epigenetic response.","description":"Ascorbic-acid addition or proline withdrawal reversed the reported proline-induced stem-cell state and reduced the associated H3K9/H3K36 methylation pattern.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0c7c34f5-86df-594c-b466-9dec4c6345c6","slug":"mouse-esc-proline-cell-state","display_name":"Proline-induced mouse embryonic-stem-cell state transition","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse embryonic stem-cell model; reversible phenotype and histone-mark measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The result does not establish a supplement ratio, a clinical methylation imbalance, or vitamin C depletion by ordinary proline intake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Vitamin C and proline intersect in this experimental epigenetic response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"L-Proline induces a mesenchymal-like invasive program in embryonic stem cells by remodeling H3K9 and H3K36 methylation. · 2013 · https://pubmed.ncbi.nlm.nih.gov/24319666/ · DOI 10.1016/j.stemcr.2013.09.001","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"880ccda2-b581-5f76-a415-b79268bb9a40","evidence_kind":"source_excerpt","locator":"Lines 398-404","start_line":398,"end_line":404,"excerpt":"## l-proline-stem-cell-vitamin-c\nVitamin C and proline intersect in this experimental epigenetic response.\nAscorbic-acid addition or proline withdrawal reversed the reported proline-induced stem-cell state and reduced the associated H3K9/H3K36 methylation pattern.\nModel: Mouse embryonic stem-cell model; reversible phenotype and histone-mark measurements.\nLimitations: The result does not establish a supplement ratio, a clinical methylation imbalance, or vitamin C depletion by ordinary proline intake.\nEvidence access: Primary abstract\nL-Proline induces a mesenchymal-like invasive program in embryonic stem cells by remodeling H3K9 and H3K36 methylation. · 2013 · https://pubmed.ncbi.nlm.nih.gov/24319666/ · DOI 10.1016/j.stemcr.2013.09.001","model_system":"Mouse embryonic stem-cell model; reversible phenotype and histone-mark measurements.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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