{"id":"69e71d68-f430-548d-8958-78bad6b77af2","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-dha-pc-oxidation","predicate":"influences-abundance-of","statement":"Vitamin E-depleted zebrafish brains contained more hydroxy-DHA-PC 38:6 than supplemented-diet controls, consistent with increased oxidation of DHA-containing phosphatidylcholine.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0f4e11f8-14e4-5fa9-bc15-b25ee48cd372","mechanism_event_label":"An oxidized DHA-containing lipid accumulated in the vitamin E-depleted brains.","subject":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"object":{"id":"006f4076-107b-52f3-9669-189dea886811","slug":"hydroxy-dha-pc-38-6","display_name":"Hydroxy-DHA-PC 38:6","entity_type_key":"lipid"},"evidence_count":1,"mechanism_event":{"id":"0f4e11f8-14e4-5fa9-bc15-b25ee48cd372","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-dha-pc-oxidation-event","event_type":"observed_intervention","label":"An oxidized DHA-containing lipid accumulated in the vitamin E-depleted brains.","description":"Vitamin E-depleted zebrafish brains contained more hydroxy-DHA-PC 38:6 than supplemented-diet controls, consistent with increased oxidation of DHA-containing phosphatidylcholine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6a008748-55ad-5eef-9ca2-73c14b7fe4a3","slug":"rrr-alpha-tocopheryl-acetate","display_name":"RRR-alpha-tocopheryl acetate","entity_type_key":"small_molecule"},"role":"dietary form","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"033a2800-fb71-5405-a144-a8e225bf456b","slug":"pc-16-0-22-6","display_name":"PC(16:0/22:6)","entity_type_key":"lipid"},"role":"DHA phospholipid substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"006f4076-107b-52f3-9669-189dea886811","slug":"hydroxy-dha-pc-38-6","display_name":"Hydroxy-DHA-PC 38:6","entity_type_key":"lipid"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Defined-diet comparison and brain lipidomics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"9 months; no added E versus 500 mg RRR-alpha-tocopheryl acetate/kg.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Adult zebrafish dietary comparison; lipid abundance and oxidation product measurements do not by themselves prove causal remodeling flux or human neuroprotection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Danio rerio","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An oxidized DHA-containing lipid accumulated in the vitamin E-depleted brains.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ver-choi2015] Novel function of vitamin E in regulation of zebrafish (Danio rerio) brain lysophospholipids discovered using lipidomics. (2015). https://pubmed.ncbi.nlm.nih.gov/25855633/ DOI: 10.1194/jlr.m058941","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Adult brain","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"edd56ffb-52c1-5108-be8c-9b3430d3fc64","evidence_kind":"source_excerpt","locator":"Lines 746-758","start_line":746,"end_line":758,"excerpt":"### ver-dha-pc-oxidation\nVitamin E-depleted zebrafish brains contained more hydroxy-DHA-PC 38:6 than supplemented-diet controls, consistent with increased oxidation of DHA-containing phosphatidylcholine.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An oxidized DHA-containing lipid accumulated in the vitamin E-depleted brains.\norganism: Danio rerio\ntissue_or_cell_type: Adult brain\nexperimental_model: Defined-diet comparison and brain lipidomics\nlimitations: Adult zebrafish dietary comparison; lipid abundance and oxidation product measurements do not by themselves prove causal remodeling flux or human neuroprotection.\nexposure: 9 months; no added E versus 500 mg RRR-alpha-tocopheryl acetate/kg.\ncross_nutrient: true\nevidence_location: Primary abstract\n[ver-choi2015] Novel function of vitamin E in regulation of zebrafish (Danio rerio) brain lysophospholipids discovered using lipidomics. 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