{"id":"69ae8685-dabf-57db-a8e0-a0c34962406a","stable_key":"research:selenoh-increases-glutathione-gene-expression","predicate":"increases","statement":"Human SELENOH overexpression increased glutathione-synthesis gene expression in murine HT22 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"ea7506fe-7bef-54d9-8b12-9b867ffa442f","mechanism_event_label":"Extra SELENOH increased expression of glutathione-building genes in these cells.","subject":{"id":"207e9d1c-e66d-5349-ac6a-ef1ca1b31a32","slug":"selenoh","display_name":"SELENOH","entity_type_key":"protein"},"object":{"id":"b34edd7f-c792-53b8-ab98-dcb495ce1df1","slug":"glutathione-synthesis-gene-expression","display_name":"Glutathione-synthesis gene expression","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ea7506fe-7bef-54d9-8b12-9b867ffa442f","stable_key":"research:selenoh-increases-glutathione-gene-expression","event_type":"expression-perturbation","label":"Extra SELENOH increased expression of glutathione-building genes in these cells.","description":"Human SELENOH overexpression increased glutathione-synthesis gene expression in murine HT22 cells.","status":"active","compartment":{"slug":"nucleus","display_name":"Nucleus"},"participants":[{"entity":{"id":"207e9d1c-e66d-5349-ac6a-ef1ca1b31a32","slug":"selenoh","display_name":"SELENOH","entity_type_key":"protein"},"role":"perturbed-protein","stoichiometry":null,"state_label":"overexpressed","sequence_order":0,"notes":""},{"entity":{"id":"b34edd7f-c792-53b8-ab98-dcb495ce1df1","slug":"glutathione-synthesis-gene-expression","display_name":"Glutathione-synthesis gene expression","entity_type_key":"cellular_process"},"role":"measured-process","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"HT22 hippocampal cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not prove an equivalent response to dietary selenium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human protein in mouse cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3d393f8b-cc8c-5884-81f3-db9180e994cd","evidence_kind":"curated_literature_summary","locator":"lines 870-880","start_line":870,"end_line":880,"excerpt":"## selenoh-increases-glutathione-gene-expression\n\nExtra SELENOH increased expression of glutathione-building genes in these cells.\n\nHuman SELENOH overexpression increased glutathione-synthesis gene expression in murine HT22 cells.\n\nOrganism: human protein in mouse cells\nCell type: HT22 hippocampal cells\nExperimental model: Overexpression\nLimitations: Does not prove an equivalent response to dietary selenium.\nPrimary reference: [Selenoprotein H is a redox-sensing high mobility group family DNA-binding protein that up-regulates genes involved in glutathione synthesis and phase II detoxification](https://pubmed.ncbi.nlm.nih.gov/17526492/)","model_system":"Overexpression","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"transcriptional-redox-regulation","plain_language":"Extra SELENOH increased expression of glutathione-building genes in these cells.","evidence_scope":"direct_experimental","papers":[{"key":"panee-2007-selenoh-dna","title":"Selenoprotein H is a redox-sensing high mobility group family DNA-binding protein that up-regulates genes involved in glutathione synthesis and phase II detoxification","url":"https://pubmed.ncbi.nlm.nih.gov/17526492/","doi":"10.1074/jbc.M702267200","year":2007,"model":"Human SELENOH expression in murine HT22 cells and DNA-binding assays","summary":"Tests redox-responsive DNA association and changes in glutathione-related gene expression."}]}}