{"id":"6959788c-1ac6-5efd-a9b4-722f899cbc97","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-tcell-glutamine-carbon","predicate":"supplies_carbon_to","statement":"Carbon-13 glutamine tracing during mouse Listeria infection showed substantial glutamine contribution to aspartate and pyrimidine synthesis in early effector CD8 T cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7b7bbd37-7bf0-5347-9e06-354b66fd888c","mechanism_event_label":"The immune cell used one amino acid to help supply another and build nucleotides.","subject":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"object":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"7b7bbd37-7bf0-5347-9e06-354b66fd888c","stable_key":"e37461ea-ea5d-5e2c-8091-138305f6dd70:l-aspartate-tcell-glutamine-carbon-event","event_type":"observed_relationship","label":"The immune cell used one amino acid to help supply another and build nucleotides.","description":"Carbon-13 glutamine tracing during mouse Listeria infection showed substantial glutamine contribution to aspartate and pyrimidine synthesis in early effector CD8 T cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bd15dd76-31ee-5c52-9180-c11de03fa913","slug":"mouse-got1","display_name":"Mouse cytosolic aspartate aminotransferase / Got1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"431e4b6a-9b26-5b2d-bf22-45e14a1f581a","slug":"mouse-got2","display_name":"Mouse mitochondrial aspartate aminotransferase / Got2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse in vivo tracer infusions and sorted effector T cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Fuel use changed across infection stages; no generalized human glutamine/aspartate supplementation benefit was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The immune cell used one amino acid to help supply another and build nucleotides.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"13C metabolite tracing reveals glutamine and acetate as critical in vivo fuels for CD8 T cells. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38809979/ · DOI 10.1126/sciadv.adj1431","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c6bc2233-8dc5-507e-a4b5-175bb75b5c5f","evidence_kind":"source_excerpt","locator":"Lines 274-280","start_line":274,"end_line":280,"excerpt":"## l-aspartate-tcell-glutamine-carbon\nThe immune cell used one amino acid to help supply another and build nucleotides.\nCarbon-13 glutamine tracing during mouse Listeria infection showed substantial glutamine contribution to aspartate and pyrimidine synthesis in early effector CD8 T cells.\nModel: Mouse in vivo tracer infusions and sorted effector T cells.\nLimitations: Fuel use changed across infection stages; no generalized human glutamine/aspartate supplementation benefit was tested.\nEvidence access: Primary full text\n13C metabolite tracing reveals glutamine and acetate as critical in vivo fuels for CD8 T cells. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38809979/ · DOI 10.1126/sciadv.adj1431","model_system":"Mouse in vivo tracer infusions and sorted effector T cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"67970dcb-34e0-5b0a-8c86-d8c3cc183444","stable_key":"import-e37461ea-ea5d-5e2c-8091-138305f6dd70","title":"L-Aspartate: redox transfer, nitrogen partitioning and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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