{"id":"68fa9b5f-19d2-54e4-8652-1b0b6bd69116","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-ogdh-stimulation-requires-thdp","predicate":"stimulates","statement":"Mg increased purified pig-heart KGDHC activity only when ThDP was present in the assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0ff88547-c573-59c0-a7e9-e7f31e180cc8","mechanism_event_label":"Magnesium and the B1-derived cofactor jointly supported maximal enzyme activity.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"8d8cf36b-d1f4-57eb-8ce2-798d6f3c30b9","slug":"oxoglutarate-dehydrogenase-complex","display_name":"2-Oxoglutarate dehydrogenase complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"0ff88547-c573-59c0-a7e9-e7f31e180cc8","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-ogdh-stimulation-requires-thdp-event","event_type":"biochemical_relationship","label":"Magnesium and the B1-derived cofactor jointly supported maximal enzyme activity.","description":"Mg increased purified pig-heart KGDHC activity only when ThDP was present in the assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"role":"required for observed Mg stimulation","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8d8cf36b-d1f4-57eb-8ce2-798d6f3c30b9","slug":"oxoglutarate-dehydrogenase-complex","display_name":"2-Oxoglutarate dehydrogenase complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Magnesium and thiamine-derived ThDP intersect at mitochondrial carbon metabolism; purified-enzyme responses do not measure whole-body energy supply.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified pig-heart KGDHC, controlled free divalent-ion and cofactor concentrations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Absence of added cofactor does not establish that all tightly bound cofactor was removed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Sus scrofa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Magnesium and the B1-derived cofactor jointly supported maximal enzyme activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[panov-1996-ogdh] Independent modulation of the activity of alpha-ketoglutarate dehydrogenase complex by Ca2+ and Mg2+ (1996). https://pubmed.ncbi.nlm.nih.gov/8555212/ DOI: 10.1021/bi952101t","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified heart mitochondrial enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e3576d5f-8d2e-582b-82d7-294a1d3c0469","evidence_kind":"source_excerpt","locator":"Lines 675-685","start_line":675,"end_line":685,"excerpt":"### mg-ogdh-stimulation-requires-thdp\nMg increased purified pig-heart KGDHC activity only when ThDP was present in the assay.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Magnesium and the B1-derived cofactor jointly supported maximal enzyme activity.\norganism: Sus scrofa\ntissue_or_cell_type: Purified heart mitochondrial enzyme\nexperimental_model: Purified pig-heart KGDHC, controlled free divalent-ion and cofactor concentrations.\nlimitations: Absence of added cofactor does not establish that all tightly bound cofactor was removed.\ncross_nutrient: Magnesium and thiamine-derived ThDP intersect at mitochondrial carbon metabolism; purified-enzyme responses do not measure whole-body energy supply.\n[panov-1996-ogdh] Independent modulation of the activity of alpha-ketoglutarate dehydrogenase complex by Ca2+ and Mg2+ (1996). https://pubmed.ncbi.nlm.nih.gov/8555212/ DOI: 10.1021/bi952101t","model_system":"Purified pig-heart KGDHC, controlled free divalent-ion and cofactor concentrations.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [panov-1996-ogdh] Independent modulation of the activity of alpha-ketoglutarate dehydrogenase complex by Ca2+ and Mg2+ (1996). https://pubmed.ncbi.nlm.nih.gov/8555212/ DOI: 10.1021/bi952101t","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}