{"id":"68a7fb93-482b-5ad2-a16d-5fc4acfdbd8a","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-calprotectin-silencing","predicate":"silencing_impairs","statement":"Combined S100A8/S100A9 siRNA increased the intracellular bacterial load in butyrate-differentiated human macrophages.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"807e22ef-e9b1-554a-92d5-5d7204e93a24","mechanism_event_label":"Reducing both components weakened the antimicrobial response.","subject":{"id":"82cffe49-2ea1-5134-9ffe-14fcc85527c7","slug":"human-calprotectin","display_name":"Human S100A8/S100A9 calprotectin complex","entity_type_key":"protein_complex"},"object":{"id":"d42547da-74a7-5b3a-9e0e-dfa4f1540079","slug":"human-butyrate-macrophage-killing","display_name":"Antimicrobial activity of butyrate-differentiated human macrophages","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"807e22ef-e9b1-554a-92d5-5d7204e93a24","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-calprotectin-silencing-event","event_type":"observed_relationship","label":"Reducing both components weakened the antimicrobial response.","description":"Combined S100A8/S100A9 siRNA increased the intracellular bacterial load in butyrate-differentiated human macrophages.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"82cffe49-2ea1-5134-9ffe-14fcc85527c7","slug":"human-calprotectin","display_name":"Human S100A8/S100A9 calprotectin complex","entity_type_key":"protein_complex"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d42547da-74a7-5b3a-9e0e-dfa4f1540079","slug":"human-butyrate-macrophage-killing","display_name":"Antimicrobial activity of butyrate-differentiated human macrophages","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a5c46289-b825-565f-972c-845739c18ecb","slug":"s100a8","display_name":"Human S100A8 / calgranulin A","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fbb3bbe5-9e26-5e79-835d-da93f18430d4","slug":"s100a9","display_name":"Human S100A9 / calgranulin B","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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