{"id":"688e1c7d-9897-5677-8e22-9a66032b8bf4","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-electron-acceptor-aspartate","predicate":"requires_electron_acceptor_for","statement":"Respiration-deficient proliferating cells became limited in aspartate synthesis; alpha-ketobutyrate restored proliferation as an electron acceptor without supplying carbon or ATP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c676c318-e144-5649-a53b-5c92811df6bf","mechanism_event_label":"Respiration supports building material by restoring electron acceptors, as well as producing ATP.","subject":{"id":"cc588cdb-0d4c-5fcb-84e1-d5589f120575","slug":"respiration-deficient-proliferating-cells","display_name":"Respiration-deficient proliferating cell models","entity_type_key":"cellular_process"},"object":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"c676c318-e144-5649-a53b-5c92811df6bf","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-electron-acceptor-aspartate-event","event_type":"observed_relationship","label":"Respiration supports building material by restoring electron acceptors, as well as producing ATP.","description":"Respiration-deficient proliferating cells became limited in aspartate synthesis; alpha-ketobutyrate restored proliferation as an electron acceptor without supplying carbon or ATP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cc588cdb-0d4c-5fcb-84e1-d5589f120575","slug":"respiration-deficient-proliferating-cells","display_name":"Respiration-deficient proliferating cell models","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"dee03e61-050d-5bde-ad0a-7f9652799ec8","slug":"alpha-ketobutyrate","display_name":"Alpha-ketobutyrate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured proliferating mammalian cells with impaired respiration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"ATP is not generally dispensable; this experiment isolates an electron-acceptor bottleneck in supplied culture conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Respiration supports building material by restoring electron acceptors, as well as producing ATP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Supporting Aspartate Biosynthesis Is an Essential Function of Respiration in Proliferating Cells. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26232225/ · DOI 10.1016/j.cell.2015.07.017","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"68ff998e-38fb-5884-a45f-ca5a5021b851","evidence_kind":"source_excerpt","locator":"Lines 204-210","start_line":204,"end_line":210,"excerpt":"## nad-plus-electron-acceptor-aspartate\nRespiration supports building material by restoring electron acceptors, as well as producing ATP.\nRespiration-deficient proliferating cells became limited in aspartate synthesis; alpha-ketobutyrate restored proliferation as an electron acceptor without supplying carbon or ATP.\nModel: Cultured proliferating mammalian cells with impaired respiration.\nLimitations: ATP is not generally dispensable; this experiment isolates an electron-acceptor bottleneck in supplied culture conditions.\nEvidence access: Primary full text\nSupporting Aspartate Biosynthesis Is an Essential Function of Respiration in Proliferating Cells. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26232225/ · DOI 10.1016/j.cell.2015.07.017","model_system":"Cultured proliferating mammalian cells with impaired respiration.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f59aa302-d463-5462-876f-e21e64861eac","stable_key":"import-8c946991-97f0-55ee-8df0-00e67621962f","title":"NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"9f5324d0d652c80ad630b3100e28ce34cd2ce260ea2e1324332a4262ce72ae09","revision_id":"0e07909c-e8bc-5950-bafc-61371d9d6412","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}