{"id":"685d942d-34c1-5756-bdda-852e847e622b","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-human-ddo-nmda","predicate":"oxidizes","statement":"Human DDO-1 also oxidized N-methyl-D-aspartate, with reported Km 6.8 mM and kcat 37.7 per second.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ff68a653-c9df-5c7e-8fea-0ad34a12003d","mechanism_event_label":"The enzyme accepts a related chemical that is distinct from the NMDA receptor.","subject":{"id":"17871951-099f-5a48-832e-ff9cb1c65755","slug":"ddo","display_name":"Human D-aspartate oxidase / DDO","entity_type_key":"protein"},"object":{"id":"4405c054-31e8-5d20-8593-e37181234e3c","slug":"n-methyl-d-aspartate","display_name":"N-Methyl-D-aspartate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"ff68a653-c9df-5c7e-8fea-0ad34a12003d","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-human-ddo-nmda-event","event_type":"observed_relationship","label":"The enzyme accepts a related chemical that is distinct from the NMDA receptor.","description":"Human DDO-1 also oxidized N-methyl-D-aspartate, with reported Km 6.8 mM and kcat 37.7 per second.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"17871951-099f-5a48-832e-ff9cb1c65755","slug":"ddo","display_name":"Human D-aspartate oxidase / DDO","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4405c054-31e8-5d20-8593-e37181234e3c","slug":"n-methyl-d-aspartate","display_name":"N-Methyl-D-aspartate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant human DDO-1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish endogenous NMDA synthesis or justify conflating NMDA with D-aspartate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The enzyme accepts a related chemical that is distinct from the NMDA receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structural and functional characterization of the human brain D-aspartate oxidase. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9163533/ · DOI 10.1093/oxfordjournals.jbchem.a021655","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7fa072ad-5083-5232-af1f-3f42e61ebc78","evidence_kind":"source_excerpt","locator":"Lines 80-86","start_line":80,"end_line":86,"excerpt":"## d-aspartate-human-ddo-nmda\nThe enzyme accepts a related chemical that is distinct from the NMDA receptor.\nHuman DDO-1 also oxidized N-methyl-D-aspartate, with reported Km 6.8 mM and kcat 37.7 per second.\nModel: Purified recombinant human DDO-1.\nLimitations: Does not establish endogenous NMDA synthesis or justify conflating NMDA with D-aspartate.\nEvidence access: Primary abstract\nStructural and functional characterization of the human brain D-aspartate oxidase. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9163533/ · DOI 10.1093/oxfordjournals.jbchem.a021655","model_system":"Purified recombinant human DDO-1.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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