{"id":"683cbed6-ebfa-5847-98ae-2c99f357ed01","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-osa-high-exposure","predicate":"reduces","statement":"At 1,700 micromolar orthosilicic acid, Saos-2 colony survival was about 20% below control, with reduced proliferation/adhesion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4365d60e-14fb-5db4-8e4b-abd0d2f56432","mechanism_event_label":"Higher exposure was not uniformly beneficial.","subject":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"object":{"id":"e1d23354-d41c-518b-84e8-4c48c378c918","slug":"human-saos2-survival","display_name":"Human Saos-2 colony survival","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4365d60e-14fb-5db4-8e4b-abd0d2f56432","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-osa-high-exposure-event","event_type":"observed_relationship","label":"Higher exposure was not uniformly beneficial.","description":"At 1,700 micromolar orthosilicic acid, Saos-2 colony survival was about 20% below control, with reduced proliferation/adhesion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c497144d-1e90-5176-a475-2d715992f5b0","slug":"orthosilicic-acid","display_name":"Orthosilicic acid / Si(OH)4","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e1d23354-d41c-518b-84e8-4c48c378c918","slug":"human-saos2-survival","display_name":"Human Saos-2 colony survival","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human osteoblast-like culture comparison, up to solubility limit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different concentration and endpoints from low-dose collagen studies; not a fabricated contradiction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Higher exposure was not uniformly beneficial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Divergent effects of orthosilicic acid and dimethylsilanediol on cell survival and adhesion in human osteoblast-like cells. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18755397/ · DOI 10.1016/j.jtemb.2008.02.001","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"14ba76f2-887d-55fe-9cd3-3df789977105","evidence_kind":"source_excerpt","locator":"Lines 200-206","start_line":200,"end_line":206,"excerpt":"## silica-osa-high-exposure\nHigher exposure was not uniformly beneficial.\nAt 1,700 micromolar orthosilicic acid, Saos-2 colony survival was about 20% below control, with reduced proliferation/adhesion.\nModel: Human osteoblast-like culture comparison, up to solubility limit.\nLimitations: Different concentration and endpoints from low-dose collagen studies; not a fabricated contradiction.\nEvidence access: Primary abstract\nDivergent effects of orthosilicic acid and dimethylsilanediol on cell survival and adhesion in human osteoblast-like cells. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18755397/ · DOI 10.1016/j.jtemb.2008.02.001","model_system":"Human osteoblast-like culture comparison, up to solubility limit.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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