{"id":"67f27658-3dbb-5cb2-90a8-f3eec7f27d78","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:calcium-activates-casr","predicate":"activates","statement":"Extracellular calcium activates cloned bovine CaSR expressed in Xenopus oocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"8b628d00-4d27-577d-b1be-a20352322df3","mechanism_event_label":"CaSR senses calcium outside cells.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"1c0d5c58-d358-5501-9bdf-8999bcf79fd4","slug":"casr","display_name":"Calcium-sensing receptor / CaSR","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8b628d00-4d27-577d-b1be-a20352322df3","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:calcium-activates-casr-event","event_type":"biochemical_relationship","label":"CaSR senses calcium outside cells.","description":"Extracellular calcium activates cloned bovine CaSR expressed in Xenopus oocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"extracellular_ligand","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1c0d5c58-d358-5501-9bdf-8999bcf79fd4","slug":"casr","display_name":"Calcium-sensing receptor / CaSR","entity_type_key":"protein"},"role":"receptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Bovine parathyroid receptor cDNA; Xenopus oocyte expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Heterologous expression; polyvalent ions also activate this receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Bos taurus receptor in Xenopus laevis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CaSR senses calcium outside cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[brown1993] Cloning and characterization of an extracellular Ca(2+)-sensing receptor from bovine parathyroid (1993). https://pubmed.ncbi.nlm.nih.gov/8255296/ DOI: 10.1038/366575a0","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Parathyroid-derived receptor; plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a51077d9-a5d9-59d7-a224-7920c9917afc","evidence_kind":"source_excerpt","locator":"Lines 14-23","start_line":14,"end_line":23,"excerpt":"### calcium-activates-casr\nExtracellular calcium activates cloned bovine CaSR expressed in Xenopus oocytes.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CaSR senses calcium outside cells.\norganism: Bos taurus receptor in Xenopus laevis\ntissue_or_cell_type: Parathyroid-derived receptor; plasma membrane\nexperimental_model: Bovine parathyroid receptor cDNA; Xenopus oocyte expression\nlimitations: Heterologous expression; polyvalent ions also activate this receptor.\n[brown1993] Cloning and characterization of an extracellular Ca(2+)-sensing receptor from bovine parathyroid (1993). https://pubmed.ncbi.nlm.nih.gov/8255296/ DOI: 10.1038/366575a0","model_system":"Bovine parathyroid receptor cDNA; Xenopus oocyte expression","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [brown1993] Cloning and characterization of an extracellular Ca(2+)-sensing receptor from bovine parathyroid (1993). https://pubmed.ncbi.nlm.nih.gov/8255296/ DOI: 10.1038/366575a0","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}