{"id":"67b30d87-a964-5c26-9696-e478668ee841","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-pgp-efflux-null","predicate":"no_detected_inhibition","statement":"Tartrazine did not inhibit human P-glycoprotein-mediated rhodamine-123 efflux in the cell assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"4b25327b-5ba5-54ac-a3cb-69b54d27486f","mechanism_event_label":"Tartrazine did not inhibit human P-glycoprotein-mediated rhodamine-123 efflux in the cell assay.","subject":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"object":{"id":"c6139b52-4140-5622-9e33-1f6c6385265d","slug":"abcb1","display_name":"Human P-glycoprotein / ABCB1 / MDR1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4b25327b-5ba5-54ac-a3cb-69b54d27486f","stable_key":"792249b7-a38d-5f43-afb6-2936818a3fa2:tartrazine-pgp-efflux-null-event","event_type":"observed_relationship","label":"Tartrazine did not inhibit human P-glycoprotein-mediated rhodamine-123 efflux in the cell assay.","description":"Tartrazine did not inhibit human P-glycoprotein-mediated rhodamine-123 efflux in the cell assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"135b5f4d-0c59-5359-a645-39796e820d42","slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c6139b52-4140-5622-9e33-1f6c6385265d","slug":"abcb1","display_name":"Human P-glycoprotein / ABCB1 / MDR1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3d400fcd-8b5b-5573-8c57-9066e8a83534","slug":"rhodamine-123","display_name":"Rhodamine 123","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Tartrazine 0.1-1000 micromolar, rhodamine-123 5 micromolar; GF120918 positive control","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"1 h at 37 C","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results and metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific experimental curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"LLC-MDR1-WT cells expressing recombinant human ABCB1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human transporter in a heterologous cell system. Negative P-gp inhibition is not inconsistent with a BCRP effect in a different assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tartrazine food-colorant chapter; nutrient, drug and peptide interactions retain their models and limits.","comparator":null,"unit":null,"notes":"","entity":{"slug":"tartrazine","display_name":"Tartrazine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"LLC-MDR1-WT cells expressing recombinant human ABCB1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Tartrazine did not inhibit human P-glycoprotein-mediated rhodamine-123 efflux in the cell assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Food dyes as P-glycoprotein modulators. (2020). https://pubmed.ncbi.nlm.nih.gov/33011351/ DOI: 10.1016/j.fct.2020.111785","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro co-incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Rhodamine-123 efflux assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"043a65ba-eb60-5911-97e8-4847799f0e8d","evidence_kind":"source_excerpt","locator":"Lines 237-246","start_line":237,"end_line":246,"excerpt":"## tartrazine-pgp-efflux-null\nTartrazine did not inhibit human P-glycoprotein-mediated rhodamine-123 efflux in the cell assay.\nModel/species: LLC-MDR1-WT cells expressing recombinant human ABCB1\nTissue: Rhodamine-123 efflux assay\nExposure: Tartrazine 0.1-1000 micromolar, rhodamine-123 5 micromolar; GF120918 positive control\nRoute: In vitro co-incubation\nDuration: 1 h at 37 C\nLimits: Human transporter in a heterologous cell system. Negative P-gp inhibition is not inconsistent with a BCRP effect in a different assay.\nPrimary reference: Food dyes as P-glycoprotein modulators. (2020). https://pubmed.ncbi.nlm.nih.gov/33011351/ DOI: 10.1016/j.fct.2020.111785\nAccess: Primary full-text methods/results and metadata.","model_system":"LLC-MDR1-WT cells expressing recombinant human ABCB1","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; primary citation retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d9540941-68b4-5a0d-a5c1-828665a918e2","stable_key":"import-792249b7-a38d-5f43-afb6-2936818a3fa2","title":"Tartrazine: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eighteen primary studies. Study-specific citations, negative findings and limitations retained. 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