{"id":"67aeec1a-2cab-54be-8d1d-27b75b798d12","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-es-protein-release","predicate":"reduces","statement":"Ivermectin exposure decreased the amount of protein released from the excretory-secretory apparatus of Brugia malayi microfilariae.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"98a5a4ba-037b-529c-94e2-2c30336ac6c6","mechanism_event_label":"Ivermectin exposure decreased the amount of protein released from the excretory-secretory apparatus of Brugia malayi microfilariae.","subject":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"object":{"id":"a1b86c4a-9525-59b9-b008-1cb134779fa6","slug":"brugia-es-protein-release","display_name":"Protein release from the Brugia malayi excretory-secretory apparatus","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"98a5a4ba-037b-529c-94e2-2c30336ac6c6","stable_key":"e0801c92-7fd3-5cb2-82b7-dd82f010f3ed:ivermectin-es-protein-release-event","event_type":"observed_relationship","label":"Ivermectin exposure decreased the amount of protein released from the excretory-secretory apparatus of Brugia malayi microfilariae.","description":"Ivermectin exposure decreased the amount of protein released from the excretory-secretory apparatus of Brugia malayi microfilariae.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"416b9886-c6a5-5526-a8bf-b571a8d418bf","slug":"ivermectin","display_name":"Ivermectin","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a1b86c4a-9525-59b9-b008-1cb134779fa6","slug":"brugia-es-protein-release","display_name":"Protein release from the Brugia malayi excretory-secretory apparatus","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fc88a4d0-ca7b-5010-9741-8ea62d0894f3","slug":"celegans-glucl","display_name":"Caenorhabditis elegans glutamate-gated chloride channel alpha / GluCl","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Acute","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. 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(2010). https://pubmed.ncbi.nlm.nih.gov/21041637/ DOI: 10.1073/pnas.1011983107","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Excretory-secretory vesicle and the muscle structure surrounding it, where GluCl expression was localised","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b8053dbe-af0c-5e69-98e9-fe67ff4e1d34","evidence_kind":"source_excerpt","locator":"Lines 35-44","start_line":35,"end_line":44,"excerpt":"## ivermectin-es-protein-release\nIvermectin exposure decreased the amount of protein released from the excretory-secretory apparatus of Brugia malayi microfilariae.\nModel/species: Brugia malayi microfilariae\nTissue/system: Excretory-secretory vesicle and the muscle structure surrounding it, where GluCl expression was localised\nExposure: Ivermectin in vitro\nRoute: In vitro\nDuration: Acute\nLimits: The link from reduced secretion to rapid microfilarial clearance in the host is proposed by the authors, not measured in this work.\nPrimary reference: Ivermectin disrupts the function of the excretory-secretory apparatus in microfilariae of Brugia malayi. (2010). https://pubmed.ncbi.nlm.nih.gov/21041637/ DOI: 10.1073/pnas.1011983107\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"20818ddd-7b37-5226-a24b-7de007e80eb2","stable_key":"import-e0801c92-7fd3-5cb2-82b7-dd82f010f3ed","title":"Ivermectin: mechanism of action across parasite, host barrier and mammalian targets (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of sixteen primary studies resolved by PubMed title search and cross-checked against live PubMed metadata. Study-specific citations, concentrations, negative findings and limitations retained. 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