{"id":"67814b76-a0f9-58b2-82ba-330f3567d5ab","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-luminal-k-enables-tal-salt-transport","predicate":"supports","statement":"Removing luminal K reduced active transport current in rabbit cortical TAL; combining K removal with luminal barium nearly abolished it.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ffb21042-eeee-595d-909e-e81e1bae212b","mechanism_event_label":"Recycled luminal potassium supports sodium and chloride uptake in the thick ascending limb.","subject":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"object":{"id":"564e0318-d850-5c5e-a94e-57ce730875b5","slug":"renal-sodium-reabsorption","display_name":"Renal sodium reabsorption","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ffb21042-eeee-595d-909e-e81e1bae212b","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:renal-luminal-k-enables-tal-salt-transport-event","event_type":"biochemical_relationship","label":"Recycled luminal potassium supports sodium and chloride uptake in the thick ascending limb.","description":"Removing luminal K reduced active transport current in rabbit cortical TAL; combining K removal with luminal barium nearly abolished it.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"causal-subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"564e0318-d850-5c5e-a94e-57ce730875b5","slug":"renal-sodium-reabsorption","display_name":"Renal sodium reabsorption","entity_type_key":"cellular_process"},"role":"measured-target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"cotransported-ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"cotransported-ion","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Luminal K availability supports coupled sodium/chloride reabsorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract; ion-removal and conductance-blocking experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated perfused tubules; K removal and barium blockade","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Electrical surrogate; the study predates molecular identification of NKCC2 and ROMK.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Oryctolagus cuniculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Recycled luminal potassium supports sodium and chloride uptake in the thick ascending limb.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[greger-1981-luminal-k] Presence of luminal K+, a prerequisite for active NaCl transport in the cortical thick ascending limb of Henle's loop of rabbit kidney (1981). https://pubmed.ncbi.nlm.nih.gov/7322839/ DOI: 10.1007/BF00584588","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cortical thick ascending limb","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b15c1efb-8060-5a54-b793-df378f6933db","evidence_kind":"source_excerpt","locator":"Lines 447-458","start_line":447,"end_line":458,"excerpt":"### renal-luminal-k-enables-tal-salt-transport\nRemoving luminal K reduced active transport current in rabbit cortical TAL; combining K removal with luminal barium nearly abolished it.\nCondition category: normal\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Recycled luminal potassium supports sodium and chloride uptake in the thick ascending limb.\norganism: Oryctolagus cuniculus\ntissue_or_cell_type: Cortical thick ascending limb\nexperimental_model: Isolated perfused tubules; K removal and barium blockade\nlimitations: Electrical surrogate; the study predates molecular identification of NKCC2 and ROMK.\ncross_nutrient: Luminal K availability supports coupled sodium/chloride reabsorption.\nevidence_location: Primary abstract; ion-removal and conductance-blocking experiments.\n[greger-1981-luminal-k] Presence of luminal K+, a prerequisite for active NaCl transport in the cortical thick ascending limb of Henle's loop of rabbit kidney (1981). https://pubmed.ncbi.nlm.nih.gov/7322839/ DOI: 10.1007/BF00584588","model_system":"Isolated perfused tubules; K removal and barium blockade","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [greger-1981-luminal-k] Presence of luminal K+, a prerequisite for active NaCl transport in the cortical thick ascending limb of Henle's loop of rabbit kidney (1981). https://pubmed.ncbi.nlm.nih.gov/7322839/ DOI: 10.1007/BF00584588","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}