{"id":"670b0a5d-a506-5ca1-9eab-6de3448838de","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-pih-iron-chelation","predicate":"chelates","statement":"The B6-isoniazid conjugate PIH bound iron in the study chelation assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a8492568-f6e0-5003-a3cc-52d747951749","mechanism_event_label":"The drug-B6 product also interacts with iron.","subject":{"id":"af123b94-0b58-5894-83f0-9b8e681b8fe5","slug":"pyridoxal-isonicotinoyl-hydrazone","display_name":"Pyridoxal isonicotinoyl hydrazone","entity_type_key":"small_molecule"},"object":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"evidence_count":1,"mechanism_event":{"id":"a8492568-f6e0-5003-a3cc-52d747951749","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-pih-iron-chelation-event","event_type":"biochemical_relationship","label":"The drug-B6 product also interacts with iron.","description":"The B6-isoniazid conjugate PIH bound iron in the study chelation assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"af123b94-0b58-5894-83f0-9b8e681b8fe5","slug":"pyridoxal-isonicotinoyl-hydrazone","display_name":"Pyridoxal isonicotinoyl hydrazone","entity_type_key":"small_molecule"},"role":"chelator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"bound-element","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Drug-modified B6 chemistry creates an iron-binding molecule.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free chemistry, primary human hepatocytes and HepG2/C3A cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Chrome azurol S assay; biochemical and cell-medium samples.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This endpoint does not establish nutritional iron deficiency in patients.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Not applicable; chemical reaction","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The drug-B6 product also interacts with iron.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[brewer-2019-isoniazid] The Isoniazid Metabolites Hydrazine and Pyridoxal Isonicotinoyl Hydrazone Modulate Heme Biosynthesis (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6390808/ DOI: 10.1093/toxsci/kfy294","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free chelation assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"be1e4393-d645-56d1-8079-c6bd50c5690e","evidence_kind":"source_excerpt","locator":"Lines 1283-1294","start_line":1283,"end_line":1294,"excerpt":"### b6-neuro-pih-iron-chelation\nThe B6-isoniazid conjugate PIH bound iron in the study chelation assay.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The drug-B6 product also interacts with iron.\norganism: Not applicable; chemical reaction\ntissue_or_cell_type: Cell-free chelation assay\nexperimental_model: Cell-free chemistry, primary human hepatocytes and HepG2/C3A cells\nlimitations: This endpoint does not establish nutritional iron deficiency in patients.\nexposure: Chrome azurol S assay; biochemical and cell-medium samples.\ncross_nutrient: Drug-modified B6 chemistry creates an iron-binding molecule.\n[brewer-2019-isoniazid] The Isoniazid Metabolites Hydrazine and Pyridoxal Isonicotinoyl Hydrazone Modulate Heme Biosynthesis (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6390808/ DOI: 10.1093/toxsci/kfy294","model_system":"Cell-free chemistry, primary human hepatocytes and HepG2/C3A cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [brewer-2019-isoniazid] The Isoniazid Metabolites Hydrazine and Pyridoxal Isonicotinoyl Hydrazone Modulate Heme Biosynthesis (2019). https://pmc.ncbi.nlm.nih.gov/articles/PMC6390808/ DOI: 10.1093/toxsci/kfy294","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}