{"id":"66e42ea2-1c90-51df-9a8a-929e527d0203","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-pdac-collagen-fuel","predicate":"supply","statement":"Human pancreatic cancer cells took up collagen fragments and used collagen-derived proline in metabolism under nutrient-limited culture conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"aaf1488e-e7b5-589b-b11f-acd1ec80c7c2","mechanism_event_label":"A tissue matrix can become a nutrient reservoir for cells.","subject":{"id":"0cf232e8-346d-54d2-97ee-659df9edaddb","slug":"collagen-proline-residues","display_name":"Collagen-bound proline residues","entity_type_key":"protein_state"},"object":{"id":"528234e1-e3ce-520f-9b16-6862caa83208","slug":"human-pdac-collagen-proline-metabolism","display_name":"Collagen-derived proline metabolism in human pancreatic cancer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"aaf1488e-e7b5-589b-b11f-acd1ec80c7c2","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-pdac-collagen-fuel-event","event_type":"observed_relationship","label":"A tissue matrix can become a nutrient reservoir for cells.","description":"Human pancreatic cancer cells took up collagen fragments and used collagen-derived proline in metabolism under nutrient-limited culture conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0cf232e8-346d-54d2-97ee-659df9edaddb","slug":"collagen-proline-residues","display_name":"Collagen-bound proline residues","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"528234e1-e3ce-520f-9b16-6862caa83208","slug":"human-pdac-collagen-proline-metabolism","display_name":"Collagen-derived proline metabolism in human pancreatic cancer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5e13a254-f92e-5317-abb5-2c683b178d39","slug":"prodh","display_name":"Human proline dehydrogenase / PRODH","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human PDAC culture and tracer experiments under limited fuel availability.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Collagen uptake, peptide digestion and proline oxidation are distinct steps; no effect of oral collagen supplements is demonstrated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A tissue matrix can become a nutrient reservoir for cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Collagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28685754/ · DOI 10.1038/ncomms16031","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f2700c9f-9da0-547c-8fa8-deea936848b0","evidence_kind":"source_excerpt","locator":"Lines 374-380","start_line":374,"end_line":380,"excerpt":"## l-proline-pdac-collagen-fuel\nA tissue matrix can become a nutrient reservoir for cells.\nHuman pancreatic cancer cells took up collagen fragments and used collagen-derived proline in metabolism under nutrient-limited culture conditions.\nModel: Human PDAC culture and tracer experiments under limited fuel availability.\nLimitations: Collagen uptake, peptide digestion and proline oxidation are distinct steps; no effect of oral collagen supplements is demonstrated.\nEvidence access: Primary full text\nCollagen-derived proline promotes pancreatic ductal adenocarcinoma cell survival under nutrient limited conditions. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28685754/ · DOI 10.1038/ncomms16031","model_system":"Human PDAC culture and tracer experiments under limited fuel availability.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e5aa7fc5-ee52-5376-8169-416082a89fd1","stable_key":"import-6612c190-1948-5bcf-bbe3-a7f6c50fa3cf","title":"L-Proline: synthesis, collagen processing, redox metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7b7f1981f9c7897fbb6baa19425bdc19dede78ad4d66fa554394b8337c7366fb","revision_id":"4971a925-fa70-59f8-8030-d3029a18a62f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}