{"id":"66a132a9-62ad-591e-870a-69ac3f54631b","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-previtamin-d2-isomerization","predicate":"thermally_isomerizes_to","statement":"Previtamin D2 generated in irradiated mushrooms converted thermally to ergocalciferol; mushroom conversion was faster than in methanol in the compared preparations.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"69a09c0d-e7f5-54f1-a700-bc386d08093a","mechanism_event_label":"The fungal photoproduct rearranges into vitamin D2.","subject":{"id":"b295beaf-0771-5f43-91bf-2c9ac30704a4","slug":"previtamin-d2","display_name":"Previtamin D2","entity_type_key":"small_molecule"},"object":{"id":"6e1cf31c-f78e-596b-b1b3-70585cf9c948","slug":"ergocalciferol","display_name":"Ergocalciferol / vitamin D2","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"69a09c0d-e7f5-54f1-a700-bc386d08093a","stable_key":"1deb434a-3547-51a0-a038-87b1ce79ec38:vd-act-previtamin-d2-isomerization-event","event_type":"biochemical_relationship","label":"The fungal photoproduct rearranges into vitamin D2.","description":"Previtamin D2 generated in irradiated mushrooms converted thermally to ergocalciferol; mushroom conversion was faster than in methanol in the compared preparations.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b295beaf-0771-5f43-91bf-2c9ac30704a4","slug":"previtamin-d2","display_name":"Previtamin D2","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6e1cf31c-f78e-596b-b1b3-70585cf9c948","slug":"ergocalciferol","display_name":"Ergocalciferol / vitamin D2","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary Results: Photoproduction of previtamin D2 and its conversion to vitamin D2; Figures 2-5.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mushroom-versus-solution HPLC time course","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Post-irradiation time course at 25 C; approximately half converted after 11.5 hours in mushrooms.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Matrix and temperature affect rate; no metabolic activation enzyme is implied.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin D2 and D3","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-d","display_name":"Vitamin D2 and D3","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Agaricus bisporus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The fungal photoproduct rearranges into vitamin D2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[keegan2013] Photobiology of vitamin D in mushrooms and its bioavailability in humans. (2013). https://pubmed.ncbi.nlm.nih.gov/24494050/ DOI: 10.4161/derm.23321","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"mushroom tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4c3f621b-ce00-5ead-b142-2cdead4bf58c","evidence_kind":"source_excerpt","locator":"Lines 151-164","start_line":151,"end_line":164,"excerpt":"### vd-act-previtamin-d2-isomerization\nPrevitamin D2 generated in irradiated mushrooms converted thermally to ergocalciferol; mushroom conversion was faster than in methanol in the compared preparations.\nCondition category: normal\nnutrient_topic: Vitamin D2 and D3 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The fungal photoproduct rearranges into vitamin D2.\norganism: Agaricus bisporus\ntissue_or_cell_type: mushroom tissue\nexperimental_model: Mushroom-versus-solution HPLC time course\nlimitations: Matrix and temperature affect rate; no metabolic activation enzyme is implied.\nexposure: Post-irradiation time course at 25 C; approximately half converted after 11.5 hours in mushrooms.\ncross_nutrient: false\nevidence_location: Primary Results: Photoproduction of previtamin D2 and its conversion to vitamin D2; Figures 2-5.\nnutrient: Vitamin D2 and D3\n[keegan2013] Photobiology of vitamin D in mushrooms and its bioavailability in humans. 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