{"id":"668327c5-3795-5c63-9951-bb5d91fde024","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-pdh-e1-thdp-anchor","predicate":"coordinates-with","statement":"Wild-type human PDH E1 structures show Mg coordinated at the ThDP diphosphate-binding site.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a617afa3-b3fa-549c-ac58-fb9f64f4e311","mechanism_event_label":"Magnesium helps position the B1-derived cofactor in the pyruvate-processing enzyme.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a617afa3-b3fa-549c-ac58-fb9f64f4e311","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-pdh-e1-thdp-anchor-event","event_type":"biochemical_relationship","label":"Magnesium helps position the B1-derived cofactor in the pyruvate-processing enzyme.","description":"Wild-type human PDH E1 structures show Mg coordinated at the ThDP diphosphate-binding site.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d2a2b619-f60a-5ace-9bcb-cdf2a6e194e6","slug":"pyruvate-dehydrogenase-e1","display_name":"Human pyruvate dehydrogenase E1","entity_type_key":"protein_complex"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"187db168-8028-5ce6-9f8b-4bc61ebad1a0","slug":"thiamine-diphosphate","display_name":"Thiamine diphosphate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Magnesium and thiamine-derived ThDP intersect at mitochondrial carbon metabolism; purified-enzyme responses do not measure whole-body energy supply.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human wild-type and alphaV138M E1 structures and reconstituted PDH complex activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structure supports binding geometry; no magnesium-deficiency intervention was performed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Magnesium helps position the B1-derived cofactor in the pyruvate-processing enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[whitley-2018-pdh] Pyruvate dehydrogenase complex deficiency is linked to regulatory loop disorder in the αV138M variant of human pyruvate dehydrogenase (2018). https://pmc.ncbi.nlm.nih.gov/articles/PMC6109939/ DOI: 10.1074/jbc.RA118.003996","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Recombinant mitochondrial enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"372a21ec-00a8-5570-a272-0d48f044ff62","evidence_kind":"source_excerpt","locator":"Lines 613-623","start_line":613,"end_line":623,"excerpt":"### mg-pdh-e1-thdp-anchor\nWild-type human PDH E1 structures show Mg coordinated at the ThDP diphosphate-binding site.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Magnesium helps position the B1-derived cofactor in the pyruvate-processing enzyme.\norganism: Homo sapiens\ntissue_or_cell_type: Recombinant mitochondrial enzyme\nexperimental_model: Recombinant human wild-type and alphaV138M E1 structures and reconstituted PDH complex activity.\nlimitations: Structure supports binding geometry; no magnesium-deficiency intervention was performed.\ncross_nutrient: Magnesium and thiamine-derived ThDP intersect at mitochondrial carbon metabolism; purified-enzyme responses do not measure whole-body energy supply.\n[whitley-2018-pdh] Pyruvate dehydrogenase complex deficiency is linked to regulatory loop disorder in the αV138M variant of human pyruvate dehydrogenase (2018). https://pmc.ncbi.nlm.nih.gov/articles/PMC6109939/ DOI: 10.1074/jbc.RA118.003996","model_system":"Recombinant human wild-type and alphaV138M E1 structures and reconstituted PDH complex activity.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [whitley-2018-pdh] Pyruvate dehydrogenase complex deficiency is linked to regulatory loop disorder in the αV138M variant of human pyruvate dehydrogenase (2018). https://pmc.ncbi.nlm.nih.gov/articles/PMC6109939/ DOI: 10.1074/jbc.RA118.003996","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}