{"id":"662d6243-906c-5a03-9356-7251df0be9ce","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-lipogenesis","predicate":"loss_reduces","statement":"Removing Crat impaired glucose-derived lipid synthesis in ACLY-deficient mouse HCC cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5af9da12-983a-5f3e-8ae6-a46cf948a444","mechanism_event_label":"The acetyl shuttle can supply lipid synthesis when a usual route is missing.","subject":{"id":"224eacc1-fea4-5737-b661-56e7e41a64e7","slug":"mouse-crat","display_name":"Mouse carnitine acetyltransferase / Crat","entity_type_key":"protein"},"object":{"id":"e6068a46-89b8-5191-9350-c7f4150dbf81","slug":"mouse-hcc-glucose-lipogenesis","display_name":"Mouse HCC-cell glucose-derived lipid synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5af9da12-983a-5f3e-8ae6-a46cf948a444","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-lipogenesis-event","event_type":"observed_relationship","label":"The acetyl shuttle can supply lipid synthesis when a usual route is missing.","description":"Removing Crat impaired glucose-derived lipid synthesis in ACLY-deficient mouse HCC cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"224eacc1-fea4-5737-b661-56e7e41a64e7","slug":"mouse-crat","display_name":"Mouse carnitine acetyltransferase / Crat","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e6068a46-89b8-5191-9350-c7f4150dbf81","slug":"mouse-hcc-glucose-lipogenesis","display_name":"Mouse HCC-cell glucose-derived lipid synthesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text and cell-line methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse HCC cell genetic perturbation and carbon tracing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Dependence arises in the specified metabolic background.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The acetyl shuttle can supply lipid synthesis when a usual route is missing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37134161/ · DOI 10.1126/sciadv.adf0115","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"add6d203-5d1b-5450-81e9-244974ce4eb1","evidence_kind":"source_excerpt","locator":"Lines 242-248","start_line":242,"end_line":248,"excerpt":"## l-carnitine-crat-lipogenesis\nThe acetyl shuttle can supply lipid synthesis when a usual route is missing.\nRemoving Crat impaired glucose-derived lipid synthesis in ACLY-deficient mouse HCC cells.\nModel: Mouse HCC cell genetic perturbation and carbon tracing.\nLimitations: Dependence arises in the specified metabolic background.\nEvidence access: Primary full text and cell-line methods\nAcetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37134161/ · DOI 10.1126/sciadv.adf0115","model_system":"Mouse HCC cell genetic perturbation and carbon tracing.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"6fcd86d9d02babb48b2ce658d506afe84580ad4b242f93f11f742d934fa5b361","revision_id":"398e3b52-45bc-52e3-891e-656c19ca674f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}