{"id":"6619b39d-4040-56bb-a522-2417387a41b8","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-pdac-carbon","predicate":"supplies_carbon","statement":"Isotope tracing showed alanine carbon entering TCA intermediates, nonessential amino acids and lipid synthesis in the tested human PDAC cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cd4cd975-253b-5674-97d2-10fd354fe827","mechanism_event_label":"The same amino acid can supply building material and mitochondrial fuel.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"85739e78-21b0-5ea7-89df-b1a2bebfa5d7","slug":"human-pdac-alanine-carbon-use","display_name":"Alanine carbon use in human pancreatic cancer cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cd4cd975-253b-5674-97d2-10fd354fe827","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-pdac-carbon-event","event_type":"observed_relationship","label":"The same amino acid can supply building material and mitochondrial fuel.","description":"Isotope tracing showed alanine carbon entering TCA intermediates, nonessential amino acids and lipid synthesis in the tested human PDAC cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"85739e78-21b0-5ea7-89df-b1a2bebfa5d7","slug":"human-pdac-alanine-carbon-use","display_name":"Alanine carbon use in human pancreatic cancer cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human pancreatic cancer cultures with labeled alanine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Tumor-cell utilization is not evidence that dietary alanine initiates cancer. Correction record: Publisher erratum corrects the second image label in Fig. 3a to hPSC-LC3 + 8988T, matching Fig. 3b; production labeling correction, not a retraction or independent study. https://www.nature.com/articles/nature19851.pdf","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same amino acid can supply building material and mitochondrial fuel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27509858/ · DOI 10.1038/nature19084","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3559e5ce-abbb-5dc9-9b66-6cc84e96f20b","evidence_kind":"source_excerpt","locator":"Lines 160-166","start_line":160,"end_line":166,"excerpt":"## alanine-pdac-carbon\nThe same amino acid can supply building material and mitochondrial fuel.\nIsotope tracing showed alanine carbon entering TCA intermediates, nonessential amino acids and lipid synthesis in the tested human PDAC cells.\nModel: Human pancreatic cancer cultures with labeled alanine.\nLimitations: Tumor-cell utilization is not evidence that dietary alanine initiates cancer. Correction record: Publisher erratum corrects the second image label in Fig. 3a to hPSC-LC3 + 8988T, matching Fig. 3b; production labeling correction, not a retraction or independent study. https://www.nature.com/articles/nature19851.pdf\nEvidence access: Primary full text\nPancreatic stellate cells support tumour metabolism through autophagic alanine secretion. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27509858/ · DOI 10.1038/nature19084","model_system":"Human pancreatic cancer cultures with labeled alanine.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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