{"id":"6612edc0-3e25-5f44-be49-85eac3ce2cbf","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-pka-blockade","predicate":"attenuates_gingerol_suppression","statement":"Adding a PKA inhibitor weakened 6-gingerol suppression of APS-IgG-triggered NETosis in human neutrophils.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"dc8d22c3-2540-5073-9ad5-02e5442ff1f1","mechanism_event_label":"Adding a PKA inhibitor weakened 6-gingerol suppression of APS-IgG-triggered NETosis in human neutrophils.","subject":{"id":"4dc352f0-38eb-59a2-8911-cee1840da9d7","slug":"human-neutrophil-pka-inhibited-state","display_name":"Pharmacologically PKA-inhibited human neutrophil state","entity_type_key":"protein_state"},"object":{"id":"8e33e68d-d8e5-5a43-b88e-83c4316515c6","slug":"human-neutrophil-netosis","display_name":"NET formation by human neutrophils","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"dc8d22c3-2540-5073-9ad5-02e5442ff1f1","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-pka-blockade-event","event_type":"observed_relationship","label":"Adding a PKA inhibitor weakened 6-gingerol suppression of APS-IgG-triggered NETosis in human neutrophils.","description":"Adding a PKA inhibitor weakened 6-gingerol suppression of APS-IgG-triggered NETosis in human neutrophils.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4dc352f0-38eb-59a2-8911-cee1840da9d7","slug":"human-neutrophil-pka-inhibited-state","display_name":"Pharmacologically PKA-inhibited human neutrophil state","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8e33e68d-d8e5-5a43-b88e-83c4316515c6","slug":"human-neutrophil-netosis","display_name":"NET formation by human neutrophils","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"837fdd65-c1eb-52e0-a724-42a1e9065c82","slug":"6-gingerol","display_name":"6-Gingerol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6cd7f461-9b79-5f75-877f-99910bb84f6c","slug":"pka","display_name":"Protein kinase A","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"dose","value_text":"6-Gingerol with APS IgG and PKA inhibitor; exact inhibitor dose not specified in inspected text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"3 h NETosis assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary open full text, results, figure legends and methods, plus PubMed metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation, not universally established human effects","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human donor neutrophils with pharmacologically inhibited PKA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Supports a PKA-sensitive component; pharmacological inhibition is not selective genetic deletion or a nutrient deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gingerols collection; each molecular form and experimental preparation remains explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gingerols","display_name":"Gingerols","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human donor neutrophils with pharmacologically inhibited PKA","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Adding a PKA inhibitor weakened 6-gingerol suppression of APS-IgG-triggered NETosis in human neutrophils.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Antineutrophil properties of natural gingerols in models of lupus. (2021). https://pubmed.ncbi.nlm.nih.gov/33373329/ DOI: 10.1172/jci.insight.138385","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Ex vivo co-exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Human neutrophils","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"00e83ccf-498d-5e15-9373-46cd45f421be","evidence_kind":"source_excerpt","locator":"Lines 481-490","start_line":481,"end_line":490,"excerpt":"## gingerols-pka-blockade\nAdding a PKA inhibitor weakened 6-gingerol suppression of APS-IgG-triggered NETosis in human neutrophils.\nModel/species: Human donor neutrophils with pharmacologically inhibited PKA\nTissue: Human neutrophils\nExposure: 6-Gingerol with APS IgG and PKA inhibitor; exact inhibitor dose not specified in inspected text\nRoute: Ex vivo co-exposure\nDuration: 3 h NETosis assay\nLimits: Supports a PKA-sensitive component; pharmacological inhibition is not selective genetic deletion or a nutrient deficiency.\nPrimary reference: Antineutrophil properties of natural gingerols in models of lupus. (2021). https://pubmed.ncbi.nlm.nih.gov/33373329/ DOI: 10.1172/jci.insight.138385\nAccess: Primary open full text, results, figure legends and methods, plus PubMed metadata.","model_system":"Human donor neutrophils with pharmacologically inhibited PKA","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact archived synthesis span with its primary citation; original paraphrase, not a claimed publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1107d60b-8e2d-55d7-8f5d-5612c49aa7dc","stable_key":"import-fd7915ff-856d-56c2-a118-4989693a7644","title":"Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. 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