{"id":"660ecfef-442c-5afe-b36a-388bede3adb0","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-katp-positive","predicate":"reduces","statement":"Stevioside at 1 micromolar reduced ATP-sensitive potassium conductance in a glucose-dependent manner in dispersed mouse beta cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b86cc263-3976-51c7-8ad0-02eca4499ffa","mechanism_event_label":"Another experiment detected a potassium-channel change at a concentration within the earlier tested range.","subject":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"object":{"id":"60d22913-5b07-537b-8954-96a0ebb6f900","slug":"mouse-beta-cell-katp-conductance","display_name":"Mouse pancreatic beta-cell ATP-sensitive potassium conductance","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b86cc263-3976-51c7-8ad0-02eca4499ffa","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-katp-positive-event","event_type":"observed_relationship","label":"Another experiment detected a potassium-channel change at a concentration within the earlier tested range.","description":"Stevioside at 1 micromolar reduced ATP-sensitive potassium conductance in a glucose-dependent manner in dispersed mouse beta cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"60d22913-5b07-537b-8954-96a0ebb6f900","slug":"mouse-beta-cell-katp-conductance","display_name":"Mouse pancreatic beta-cell ATP-sensitive potassium conductance","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Conventional and permeabilized whole-cell patch clamp of mouse islet beta cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not by itself establish direct pore binding; glucose dependence and recording conditions may explain the discrepancy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Another experiment detected a potassium-channel change at a concentration within the earlier tested range.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Rebaudioside A directly stimulates insulin secretion from pancreatic beta cells: a glucose-dependent action via inhibition of ATP-sensitive K-channels. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18435771/ · DOI 10.1111/j.1463-1326.2008.00864.x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9460d1eb-1ee1-52e1-9d72-bf4d21cdb1a1","evidence_kind":"source_excerpt","locator":"Lines 234-240","start_line":234,"end_line":240,"excerpt":"## stevia-katp-positive\nAnother experiment detected a potassium-channel change at a concentration within the earlier tested range.\nStevioside at 1 micromolar reduced ATP-sensitive potassium conductance in a glucose-dependent manner in dispersed mouse beta cells.\nModel: Conventional and permeabilized whole-cell patch clamp of mouse islet beta cells.\nLimitations: This does not by itself establish direct pore binding; glucose dependence and recording conditions may explain the discrepancy.\nEvidence access: Primary abstract\nRebaudioside A directly stimulates insulin secretion from pancreatic beta cells: a glucose-dependent action via inhibition of ATP-sensitive K-channels. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18435771/ · DOI 10.1111/j.1463-1326.2008.00864.x","model_system":"Conventional and permeabilized whole-cell patch clamp of mouse islet beta cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a36a82c2f8a0a971ea43e9e742db65f45848d13ab77310c0dd6b5cd55e31fc16","revision_id":"ea0355af-a926-511a-a7f2-1750baa95a8d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[{"id":"0e68ed5d-a299-5acc-82da-9139210fc981","title":"Stevioside and beta-cell KATP activity: no effect versus reduced conductance","kind":"contradiction","status":"open","why":"Primary reports disagree on the measured potassium-channel endpoint: the 2000 study reported no channel effect over 1–100 micromolar stevioside, while the 2008 study reported reduced conductance at 1 micromolar under high-glucose conditions. This is an apparent experimental discrepancy, not an editorial correction. Cell preparation, glucose and patch configuration may contribute, but the discrepancy has not been resolved by a matched reanalysis.","resolution":"Unresolved. Preserve both measurements; do not assert that stevioside is a direct KATP blocker. A glucose-dependent ATP/ADP mechanism and TRPM5-mediated potentiation could coexist. Full protocol comparison and a matched experiment are needed before assigning the reason.","created_at":"2026-09-20 03:56:22","record_type":"conflict","display_label":"Recorded conflict","record_url":"/conflicts/0e68ed5d-a299-5acc-82da-9139210fc981","sides":[{"conflict_id":"0e68ed5d-a299-5acc-82da-9139210fc981","ordinal":0,"label":"No detected channel effect, 2000","revision_id":"ea0355af-a926-511a-a7f2-1750baa95a8d","start_line":226,"end_line":232,"quote":"## stevia-katp-null\nOne experiment found insulin stimulation without a detected potassium-channel effect.\nStevioside at 1–100 micromolar did not alter ATP-sensitive potassium-channel activity in the reported beta-cell experiments.\nModel: Mouse islets and rat INS-1 beta-cell experiments; channel findings summarized in the primary abstract.\nLimitations: The accessed abstract includes mouse islets and rat INS-1 cells but does not unambiguously assign the channel recording to one preparation; full protocol-level reconciliation with the later positive report is incomplete.\nEvidence access: Primary abstract\nStevioside acts directly on pancreatic beta cells to secrete insulin: actions independent of cyclic adenosine monophosphate and adenosine triphosphate-sensitive K+-channel activity. · 2000 · https://pubmed.ncbi.nlm.nih.gov/10690946/ · DOI 10.1016/s0026-0495(00)91325-8","source_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","source_title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","claim_ids":["c3fccf70-17bb-52bb-aff3-50991d4186d4"]},{"conflict_id":"0e68ed5d-a299-5acc-82da-9139210fc981","ordinal":1,"label":"Reduced conductance, 2008","revision_id":"ea0355af-a926-511a-a7f2-1750baa95a8d","start_line":234,"end_line":240,"quote":"## stevia-katp-positive\nAnother experiment detected a potassium-channel change at a concentration within the earlier tested range.\nStevioside at 1 micromolar reduced ATP-sensitive potassium conductance in a glucose-dependent manner in dispersed mouse beta cells.\nModel: Conventional and permeabilized whole-cell patch clamp of mouse islet beta cells.\nLimitations: This does not by itself establish direct pore binding; glucose dependence and recording conditions may explain the discrepancy.\nEvidence access: Primary abstract\nRebaudioside A directly stimulates insulin secretion from pancreatic beta cells: a glucose-dependent action via inhibition of ATP-sensitive K-channels. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18435771/ · DOI 10.1111/j.1463-1326.2008.00864.x","source_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","source_title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","claim_ids":["660ecfef-442c-5afe-b36a-388bede3adb0"]}]}],"corrections":[],"research":null}