{"id":"65f16a17-f6eb-57b3-82cc-f419505cdc70","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-glt1-deletion","predicate":"limits","statement":"Homozygous Slc1a2/GLT-1 deletion in mice caused lethal spontaneous seizures and increased susceptibility to cortical injury, with elevated residual glutamate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"da83c156-07e6-54cf-a4c6-860aa5f6ba1d","mechanism_event_label":"Failure to clear a transmitter can be harmful even though the molecule is normally essential.","subject":{"id":"fa33b3d4-c4c4-5ec5-96f8-c56728f400b7","slug":"mouse-slc1a2","display_name":"Mouse glutamate transporter GLT-1 / Slc1a2","entity_type_key":"protein"},"object":{"id":"d04f5f8e-047d-5d6a-9c4d-45af29a94d8f","slug":"mouse-glt1-loss-seizure-injury","display_name":"Seizures and cortical-injury susceptibility in mouse GLT-1 deficiency","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"da83c156-07e6-54cf-a4c6-860aa5f6ba1d","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-glt1-deletion-event","event_type":"observed_relationship","label":"Failure to clear a transmitter can be harmful even though the molecule is normally essential.","description":"Homozygous Slc1a2/GLT-1 deletion in mice caused lethal spontaneous seizures and increased susceptibility to cortical injury, with elevated residual glutamate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fa33b3d4-c4c4-5ec5-96f8-c56728f400b7","slug":"mouse-slc1a2","display_name":"Mouse glutamate transporter GLT-1 / Slc1a2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d04f5f8e-047d-5d6a-9c4d-45af29a94d8f","slug":"mouse-glt1-loss-seizure-injury","display_name":"Seizures and cortical-injury susceptibility in mouse GLT-1 deficiency","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse whole-body transporter knockout and acute cortical injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Developmental gene deletion is not ordinary variation in dietary glutamate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Failure to clear a transmitter can be harmful even though the molecule is normally essential.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Epilepsy and exacerbation of brain injury in mice lacking the glutamate transporter GLT-1. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9180080/ · DOI 10.1126/science.276.5319.1699","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"e49ed6f9-08fd-5420-aff7-ba12e4a84ad6","evidence_kind":"source_excerpt","locator":"Lines 114-120","start_line":114,"end_line":120,"excerpt":"## glutamate-glt1-deletion\nFailure to clear a transmitter can be harmful even though the molecule is normally essential.\nHomozygous Slc1a2/GLT-1 deletion in mice caused lethal spontaneous seizures and increased susceptibility to cortical injury, with elevated residual glutamate.\nModel: Mouse whole-body transporter knockout and acute cortical injury.\nLimitations: Developmental gene deletion is not ordinary variation in dietary glutamate.\nEvidence access: Primary abstract\nEpilepsy and exacerbation of brain injury in mice lacking the glutamate transporter GLT-1. · 1997 · https://pubmed.ncbi.nlm.nih.gov/9180080/ · DOI 10.1126/science.276.5319.1699","model_system":"Mouse whole-body transporter knockout and acute cortical injury.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3da83bd75fddeb96d17af72d7e2ae04344779957650f266521f6c8ebd59fb54f","revision_id":"55196c72-94e4-540a-a869-81d35647b04a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}