{"id":"65ebf2cb-1c4c-5365-ae84-5110f8ecaac3","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-sulfite-independent","predicate":"causes","statement":"Sulfite retained cytotoxicity in cystine-free medium, and MK801 failed to rescue that component.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8c948776-f1fe-56a4-82ef-237daaba229a","mechanism_event_label":"Blocking the SSC receptor pathway does not explain or prevent every sulfite effect.","subject":{"id":"914ace7c-9176-53f7-80a9-9ee71d26ea52","slug":"sulfite","display_name":"Sulfite / SO3(2-)","entity_type_key":"ion"},"object":{"id":"2b81f4cf-eb28-5c45-94fd-650f6ee2e89c","slug":"ssc-independent-sulfite-cytotoxicity","display_name":"S-Sulfocysteine-independent sulfite cytotoxicity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8c948776-f1fe-56a4-82ef-237daaba229a","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-sulfite-independent-event","event_type":"biochemical_relationship","label":"Blocking the SSC receptor pathway does not explain or prevent every sulfite effect.","description":"Sulfite retained cytotoxicity in cystine-free medium, and MK801 failed to rescue that component.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"59569e15-8ae2-5bf8-976b-bbae0ba2abc9","slug":"mk801","display_name":"Dizocilpine / MK-801","entity_type_key":"small_molecule"},"role":"failed intervention","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"914ace7c-9176-53f7-80a9-9ee71d26ea52","slug":"sulfite","display_name":"Sulfite / SO3(2-)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b81f4cf-eb28-5c45-94fd-650f6ee2e89c","slug":"ssc-independent-sulfite-cytotoxicity","display_name":"S-Sulfocysteine-independent sulfite cytotoxicity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/29106383.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d7cfb311498ba2a88e3fc04be988dcffda740bbd1d93ada686cabf801861cf2a\", \"start_char\": 14836, \"end_char\": 15490, \"text_sha256\": \"e325c707b167b422f3b1aa7650a7dfdf7b9fca124d93bd15827b2ebbbd1ea8da\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary murine neurons, chemical reaction assays and tungstate-induced MoCD mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"SSC/sulfite exposure; receptor/calcium/calpain inhibition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mechanistic model evidence. 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Mouse drug rescue does not establish human treatment efficacy; sulfite also has SSC-independent toxicity.\nexposure: SSC/sulfite exposure; receptor/calcium/calpain inhibition\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/29106383.fulltext.txt\", \"locator\": \"Exact primary full-text span; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d7cfb311498ba2a88e3fc04be988dcffda740bbd1d93ada686cabf801861cf2a\", \"start_char\": 14836, \"end_char\": 15490, \"text_sha256\": \"e325c707b167b422f3b1aa7650a7dfdf7b9fca124d93bd15827b2ebbbd1ea8da\"}\n[mo-p29106383] S-sulfocysteine/NMDA receptor-dependent signaling underlies neurodegeneration in molybdenum cofactor deficiency. 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